Kmt2b targets both bivalent and monovalent chromatin in GSCs

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Kmt2b targets both bivalent and monovalent chromatin in GSCs Kmt2b targets both bivalent and monovalent chromatin in GSCs. (A) Generation and knockout of Kmt2bFC/FC GSCs (n=2) for molecular analysis (see Materials and Methods for details). Kmt2b targets both bivalent and monovalent chromatin in GSCs. (A) Generation and knockout of Kmt2bFC/FC GSCs (n=2) for molecular analysis (see Materials and Methods for details). (B) Average enrichment of H3K4me3 ChIP-seq reads over all TSSs±3 kb. (C) Heat map showing H3K4me3 and H3K27me3 enrichment over TSS±3 kb. All TSSs carrying an H3K4me3 peak are shown (n=15,842). Genes (rows) for all plots were ordered in the same way: by the level of H3K4me3 decline in Kmt2bFC/FC GSCs. Significantly affected genes (n=1085) are indicated (vertical line at the top right corner). (D) Heat map showing H3K4me3 and H3K27me3 enrichment in GSCs for all H3K4me3-affected genes over TSSs±2 kb (n=1085). Kmt2b-affected TSSs were subdivided into bivalent (top, n=793) and monovalent (bottom, n=292) genes (see Materials and Methods) and H3K4me3 and H3K27me3 enrichments are shown. (E) GO terms (biological processes) for bivalent and monovalent targets showing reductions in H3K4me3 levels. (F) Examples of ChIP-seq peaks at TSSs at bivalent (Dll3 and Grin2b) and monovalent (Tmem140 and Pla2g10) promoters. Affected H3K4me3 peaks at TSSs are shaded. Shin-ichi Tomizawa et al. Development 2018;145:dev169102 © 2018. Published by The Company of Biologists Ltd