Dynamic transcriptional and epigenomic reprogramming from pediatric nasal epithelial cells to induced pluripotent stem cells  Hong Ji, PhD, Xue Zhang,

Slides:



Advertisements
Similar presentations
Volume 64, Issue 5, Pages (November 2013)
Advertisements

Volume 9, Issue 1, Pages (July 2017)
Inhaled long-acting β2 agonists enhance glucocorticoid receptor nuclear translocation and efficacy in sputum macrophages in COPD  Rubaiyat Haque, MBBS,
Volume 13, Issue 2, Pages (August 2013)
Generation of Induced Pluripotent Stem Cell Lines from Adult Rat Cells
Volume 64, Issue 5, Pages (November 2013)
Weiguo Chen, MD, PhD, Yasuhiro Tabata, MD, PhD, Aaron M
Hypoxia Enhances the Generation of Induced Pluripotent Stem Cells
Regulation of GM-CSF expression by the transcription factor c-Maf
Volume 4, Issue 6, Pages (June 2009)
Umasundari Sivaprasad, PhD, David J. Askew, PhD, Mark B
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Increased TGF-β2 in severe asthma with eosinophilia
The nasal methylome and childhood atopic asthma
Histologic eosinophilic gastritis is a systemic disorder associated with blood and extragastric eosinophilia, TH2 immunity, and a unique gastric transcriptome 
Establishment of Endoderm Progenitors by SOX Transcription Factor Expression in Human Embryonic Stem Cells  Cheryle A. Séguin, Jonathan S. Draper, Andras.
Volume 3, Issue 3, Pages (September 2008)
Dissecting childhood asthma with nasal transcriptomics distinguishes subphenotypes of disease  Alex Poole, MS, Cydney Urbanek, BS, Celeste Eng, BS, Jeoffrey.
Environmental changes could enhance the biological effect of Hop J pollens on human airway epithelial cells  Seung Ihm Lee, PhD, Le Duy Pham, MD, Yoo.
Is 9 more than 2 also in allergic airway inflammation?
Patient-Specific Naturally Gene-Reverted Induced Pluripotent Stem Cells in Recessive Dystrophic Epidermolysis Bullosa  Jakub Tolar, John A. McGrath, Lily.
Advances in basic and clinical immunology in 2011
Reprogramming of T Cells from Human Peripheral Blood
Meeyoung Cho, Ph. D. , Eun Ju Lee, Ph. D. , Hyun Nam, Ph. D
Dawn C. Newcomb, PhD, Madison G
Chang Xiao, MD, PhD, Jocelyn M
Volume 9, Issue 5, Pages (November 2017)
Staphylococcus aureus enhances the tight junction barrier integrity in healthy nasal tissue, but not in nasal polyps  Can Altunbulakli, MSc, Rita Costa,
Role of p63/p73 in epithelial remodeling and their response to steroid treatment in nasal polyposis  Chun Wei Li, PhD, Li Shi, MD, Ke Ke Zhang, MD, Tian.
Volume 3, Issue 5, Pages (November 2008)
Yang Li, Ph.D., Ji-chun Tan, M.D., Ph.D., Ling-song Li, M.D., Ph.D. 
Claire A. Higgins, Munenari Itoh, Keita Inoue, Gavin D
Airway epithelial cells from asthmatic children differentially express proremodeling factors  Jesus M. Lopez-Guisa, PhD, Claire Powers, BA, Daniele File,
Ten-eleven translocation 1 (TET1) methylation is associated with childhood asthma and traffic-related air pollution  Hari K. Somineni, MS, Xue Zhang,
Volume 13, Issue 2, Pages (August 2013)
Corticosteroid-resistant asthma is associated with classical antimicrobial activation of airway macrophages  Elena Goleva, PhD, Pia J. Hauk, MD, Clifton.
Volume 6, Issue 1, Pages (January 2016)
Profiling of human CD4+ T-cell subsets identifies the TH2-specific noncoding RNA GATA3-AS1  Huan Zhang, MD, PhD, Colm E. Nestor, PhD, Shuli Zhao, PhD,
Potentiation of IL-19 expression in airway epithelia by IL-17A and IL-4/IL-13: Important implications in asthma  Fei Huang, PhD, Shinichiro Wachi, PhD,
Volume 7, Issue 1, Pages (July 2010)
Generation of neural cells using iPSCs from sleep bruxism patients with 5-HT2A polymorphism  Yurie Hoashi, D.D.S., Ph.D., Satoshi Okamoto, Ph.D., Yuka.
Antiviral activity of human β-defensin 3 against vaccinia virus
Volume 7, Issue 1, Pages 1-10 (July 2016)
Volume 7, Issue 4, Pages (October 2016)
Volume 9, Issue 5, Pages (November 2017)
Altered gene expression profiles in nasal respiratory epithelium reflect stable versus acute childhood asthma  Jesus R. Guajardo, MD, MHPE, Kathleen W.
Volume 2, Issue 3, Pages (March 2014)
Volume 13, Issue 6, Pages (December 2013)
Ten-eleven translocation 1 (TET1) methylation is associated with childhood asthma and traffic-related air pollution  Hari K. Somineni, MS, Xue Zhang,
Volume 8, Issue 5, Pages (May 2017)
Generation of Melanocytes from Induced Pluripotent Stem Cells
High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic.
Volume 3, Issue 6, Pages (December 2008)
Volume 13, Issue 3, Pages (September 2013)
Hong Ji, PhD, Gurjit K. Khurana Hershey, MD, PhD 
CC chemokine receptors CCR1 and CCR4 are expressed on airway mast cells in allergic asthma  Kawa Amin, PhD, Christer Janson, MD, PhD, Ilkka Harvima, MD,
Epigenetic Memory and Preferential Lineage-Specific Differentiation in Induced Pluripotent Stem Cells Derived from Human Pancreatic Islet Beta Cells 
Volume 7, Issue 1, Pages (July 2010)
Volume 3, Issue 3, Pages (September 2008)
Weiguo Chen, PhD, Gurjit K. Khurana Hershey, MD, PhD 
Jakub Tolar, Lily Xia, Megan J. Riddle, Chris J. Lees, Cindy R
Volume 4, Issue 1, Pages (January 2009)
Volume 8, Issue 5, Pages (May 2017)
Autophagy: Nobel Prize 2016 and allergy and asthma research
Embryonic and adult stem cell therapy
Volume 21, Issue 1, Pages (January 2013)
Nanog-Independent Reprogramming to iPSCs with Canonical Factors
Forkhead box protein 3 (FOXP3) hypermethylation is associated with diesel exhaust exposure and risk for childhood asthma  Kelly J. Brunst, PhD, Yuet-Kin.
Douglas A. Kuperman, PhD, Christina C. Lewis, PhD, Prescott G
Natural history of cow’s milk allergy
Presentation transcript:

Dynamic transcriptional and epigenomic reprogramming from pediatric nasal epithelial cells to induced pluripotent stem cells  Hong Ji, PhD, Xue Zhang, PhD, Sunghee Oh, PhD, Christopher N. Mayhew, PhD, Ashley Ulm, BA, Hari K. Somineni, MS, Mark Ericksen, BS, James M. Wells, PhD, Gurjit K. Khurana Hershey, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 135, Issue 1, Pages 236-244 (January 2015) DOI: 10.1016/j.jaci.2014.08.038 Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Reprogramming of pediatric NECs. A, Timeline and key steps for reprogramming. B, Representative morphology of NECs before transduction and ESC-like colonies after transduction. hESCs and NEC-iPSCs before (top; scale bar = 500 μm) and after (bottom; scale bar = 100 μm) transition to feeder-free culture are also shown. C, SPT cocktail enhances reprogramming efficiency. Purple color indicates alkaline phosphatase staining. Journal of Allergy and Clinical Immunology 2015 135, 236-244DOI: (10.1016/j.jaci.2014.08.038) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 NEC-iPSCs express pluripotency markers through downregulation of promoter methylation. A, Nuclear expression of Oct4 and Tra-1-60 in NEC-iPSCs. DAPI, 4′-6-Diamidino-2-phenylindole dihydrochloride. B, Expression of NANOG and CK19 in ESCs, NEC-iPSCs, and nasal epithelial samples (NE). GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. C, Promoter methylation of OCT4 and NANOG in samples from Fig 2, B. Data represent means ± SDs from 3 independent experiments of 2 biological samples. One-way ANOVA: **P < .01 and ****P < .001. Journal of Allergy and Clinical Immunology 2015 135, 236-244DOI: (10.1016/j.jaci.2014.08.038) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 NEC-iPSCs can differentiate into 3 germ layers in vitro and in vivo. A, Expression of pluripotency and germ layer markers in NEC-iPSCs (Undiff.) and embryonic bodies (EB). GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. B, In vivo differentiation of NEC-iPSCs in teratomas. Tissues were derived from 3 embryonic germ layers: glandular epithelia (g; endoderm), cartilage (c; mesoderm), and pigmented neuroepithelium (ne; ectoderm). C, G-banded karyotype analysis of NEC-iPSCs. Journal of Allergy and Clinical Immunology 2015 135, 236-244DOI: (10.1016/j.jaci.2014.08.038) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 NEC-iPSCs have similar transcriptomes compared with ESCs. A, Hierarchic clustering of ESCs, NEC-iPSCs, cNECs, and nasal epithelial samples (NEs). B, Correlation map of samples shown in Fig 4, A. Pearson coefficients between samples were plotted. C, Overlap between the differentially expressed genes from indicated comparisons. D and E, Quantitative RT-PCR of KRT5 and SPRR2A. Data represent means ± SDs of indicated samples. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. One-way ANOVA: **P < .01 and ****P < .0001. Journal of Allergy and Clinical Immunology 2015 135, 236-244DOI: (10.1016/j.jaci.2014.08.038) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 NEC-iPSCs have a similar methylome compared with that of ESCs. A, Hierarchic clustering of the same samples shown in Fig 4. B, Reprogramming of airway-specific markers KRT5, ARG2, and ANO3. Data represent means ± SDs of indicated samples. NE, Nasal epithelial samples. One-way ANOVA: ****P < .0001. Journal of Allergy and Clinical Immunology 2015 135, 236-244DOI: (10.1016/j.jaci.2014.08.038) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Epigenetic memory of parental tissue persists in NEC-iPSCs. A, DNA methylation at 20 CpG sites with epigenetic memory related to epithelial function. B-D, DNA methylation at CpG sites located within the RPTN, SPRR2A, and CAT promoters. Data represent means ± SDs of duplicate experiments for 2 or more biological samples for each indicated cell type. One-way ANOVA: *P < .05, **P < .01, ***P < .001, and ****P < .0001. Journal of Allergy and Clinical Immunology 2015 135, 236-244DOI: (10.1016/j.jaci.2014.08.038) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions