Synergistic activity of LMO-2, SCL and GATA-1 expression in whole embryos. Synergistic activity of LMO-2, SCL and GATA-1 expression in whole embryos. (Top)

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Synergistic activity of LMO-2, SCL and GATA-1 expression in whole embryos. Synergistic activity of LMO-2, SCL and GATA-1 expression in whole embryos. (Top) Embryos were injected at the one cell stage with synthetic mRNAs encoding SCL and LMO-2 (1 ng). Embryos were cultured to stage 33 and stained for tadpole α-globin protein by immunohistochemistry. (Bottom) Tadpoles injected with SCL, LMO-2 or GATA-1 alone develop normally and globin expression is limited to the ventral blood island (VBI, black arrows). Combination of SCL and LMO-2 mRNA results in developmental abnormalities. Head structures are diminished or completely missing and globin expression extends laterally around the embryo, reaching at least half way up the body. Co-injection of LMO-2, SCL and GATA-1 results in severe developmental abnormalities: dorsal/anterior structures are absent and globin staining is evident throughout the body axis. Paul E. Mead et al. Development 2001;128:2301-2308 © 2001.