Phylogenetic tree of 38 Pseudomonas type strains, based on a concatenated nine-gene MLST analysis. Phylogenetic tree of 38 Pseudomonas type strains, based.

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Phylogenetic tree of 38 Pseudomonas type strains, based on a concatenated nine-gene MLST analysis. Phylogenetic tree of 38 Pseudomonas type strains, based on a concatenated nine-gene MLST analysis. The strains selected have full-genome sequences available through public databases. The MLST analysis was performed using nine housekeeping genes (encoding DnaE, PpsA, RecA, RpoB, GyrB, GuaA, MutL, PyrC, and AcsA), with E. coli strain K-12 used as the outgroup. A maximum likelihood tree was calculated in the online version of MAFFT (209, 210) and visualized with the software program Archaeopteryx (211). The confidence intervals after 1,000 bootstrap resamplings are indicated in red, and the branch distances are indicated in black. The bar indicates sequence divergence. P. fluorescens clade destinations are based on those proposed previously (49). Brittan S. Scales et al. Clin. Microbiol. Rev. 2014; doi:10.1128/CMR.00044-14