Fig. 2. STK35 RNAs are expressed in distinct yet overlapping cell populations in adult mouse testis. STK35 RNAs are expressed in distinct yet overlapping.

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Fig. 2. STK35 RNAs are expressed in distinct yet overlapping cell populations in adult mouse testis. STK35 RNAs are expressed in distinct yet overlapping cell populations in adult mouse testis. (A) In situ hybridization of adult mouse testis with P4b (long mRNA), Pnc1 (Stk35os1) and P2/3 (short mRNA), antisense and sense probes. Spermatogonia, white arrowheads; pachytene spermatocytes, black chevron; round spermatids, black arrow. Scale bars: 50 µm and are representative of all images on that line, unless otherwise indicated. (B) Diagram summarizing the expression of STK35 long mRNA, Stk35os1 lncRNA and STK35 short mRNA in the major cell types of the mouse seminiferous epithelium: S, spermatogonia; PL, preleptotene; L, leptotene; Z, zygotene; P, pachytene spermatocyte; mm, meiotic divisions; R, round spermatids; El, elongating spermatids; Ed, elongated spermatids. (C) Transcript levels of STK35 long (P4b), Stk35os1 (Pnc1) and short/long (P2/3) mRNAs were analyzed by qRT-PCR on 7, 14, 28 and 60 dpp Swiss Asmu mouse testes (n=3/age). Data are presented as fold-change compared to 7 dpp (mean±s.d.). Significance determined using two-way ANOVA with Tukey's multiple comparisons test. Lowercase letters a, b and c indicate values within each graph that are significantly different (P<0.01). Yoichi Miyamoto et al. Biology Open 2018;7:bio032631 © 2018. Published by The Company of Biologists Ltd