Reduced S345 phosphorylation of Chk1-S173A in unchallenged cells

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Fig. 1. Reduced S345 phosphorylation of Chk1-S173A in unchallenged cells. Reduced S345 phosphorylation of Chk1-S173A in unchallenged cells. (A) Model of the kinase domain of S. pombe Chk1. The Swiss model tool was used (https://swissmodel.expasy.org). The underlying crystal structure is 4czt (34.5% identity) (Chaves-Sanjuan et al., 2014). Serine-321 is the last C-terminal amino acid. (B) chk1-HA3 wild-type cells were treated in rich medium with 10 μM camptothecin (CPT) for 3.5 h at 30°C. UT, untreated. Total protein extracts were separated on normal 10% SDS page or 6% phostag SDS page (PT). The arrow indicates the group of shift bands related to S345 phosphorylation. (C) PT-SDS page showing extracts from chk1-HA3, chk1-S345A-HA3 and chk1-HA3 rad3::ade6+ cells treated with 10 μM CPT for 3.5 h. Numbers 1 and 2 indicate the hypo-phosphorylated double band. (D) Normal SDS page analysis of chk1-HA3 and chk1-S173A-HA3 cells treated with 12 mM hydroxyurea (HU) and 10μM CPT for 3.5 h or with 10 µM nitroquinoline 1-oxide (4NQO) for 1 h at 30°C (full image shown in Fig. S2B) (Chk1 runs at 58 kDa). (E) PT-SDS page analysing extracts from untreated chk1-HA3, chk1-HA3 rad3::ade6+, chk1-S173A-HA3 and chk1-S173A-HA3 rad3::ade6+ cells. (F,G) Untreated chk1-HA3 and chk1-S173A-HA3 cells were grown in rich medium from a low cell number into stationary phase. Samples were withdrawn at the indicated time points and analysed on PT-SDS page. 1 and 2 indicate the Chk1 double band. The two bands labelled with 3 appear specifically in non-growing cells. Naomi Coulton, and Thomas Caspari Biology Open 2017;6:1840-1850 © 2017. Published by The Company of Biologists Ltd