Fig. 1. PolySTAT synthesis and characterization.

Slides:

Advertisements

Similar presentations

Single-Chain Nanoparticles from Sequenced Polyolefins Acknowledgments Thank you to Dr. Erik Berda and the Berda research group for allowing me to join.

Advertisements

1 Characterizing Molecular Weight Brazel & Rosen Ch. 5 Number average molecular weight: M n.

Characterization of SCNPs Summary and Conclusions

A.R. Wufsus, N.E. Macera, K.B. Neeves Biophysical Journal

Neutrophil aggregation is selectively induced by PS+ platelets

Fig. 5. Vascularization of human liver seed grafts.

Confocal images of negative controls for Aβ42, Synaptophysin (syp), and PSD95 in hippocampal neurons. Confocal images of negative controls for Aβ42, Synaptophysin.

Fig. 2 STED microscopy of isolated cardiomyocytes from mice treated with MP-rhodamine–loaded CaPs. STED microscopy of isolated cardiomyocytes from mice.

Fig. 2. Rheological characterization and hydrogel differentiation.

TCR signaling induces nuclear actin polymerization.

Fig. 6. Rolling neutrophils extract membranes from fragile remnant PS+ platelets. Rolling neutrophils extract membranes from fragile remnant PS+ platelets.

Cytosolic entry of Lm required for CD8α+ DC recruitment.

Fig. 6 Transmissibility of adiposity from humanized mice to germ-free recipients. Transmissibility of adiposity from humanized mice to germ-free recipients.

Fig. 5 In vivo MIP imaging of lipid and protein in C. elegans.

Fig. 5 Metafluorophores with different photostability.

Fig. 1 ZIKV RNA in blood and tissues.

Consolidated Standards of Reporting Trials flow diagram of VRC trial

Fig. 2. Clinically actionable somatic genomic alterations in various tumor types. Clinically actionable somatic genomic alterations in various tumor types.

Fig. 1. Schematic showing the shedding of tumor DNA from head and neck cancers into the saliva or plasma. Schematic showing the shedding of tumor DNA from.

Fig. 3. Comparison of prediction performance.

Fig. 2. The N terminus of moZP2 decoys sperm in vitro and prevents mouse fertilization. The N terminus of moZP2 decoys sperm in vitro and prevents mouse.

Effect of M-cadherin RNAi and GSK-3β inhibition on myogenic differentiation of C2C12 myoblasts. Effect of M-cadherin RNAi and GSK-3β inhibition on myogenic.

Fig. 4 Rotation of nuclei in oocytes in the primordial follicles.

Fig. 3. BET inhibition reduces homologous recombination.

HT synthesis of boronic acids using the building block approach

Fig. 2. Deficiency of neuronal HS leads to reduced neuroinflammation.

Nanoscale adhesive geometry modulates integrin–FN clustering.

Fig. 2. Bacterial/viral score in COCONUT-conormalized whole-blood validation data sets. Bacterial/viral score in COCONUT-conormalized whole-blood validation.

Fig. 5. Col IV–Ac2-26 NPs decrease lesion area, necrotic area, and oxidative stress in brachiocephalic arterial plaques. Col IV–Ac2-26 NPs decrease lesion.

Colonization in tumor models and different modes of administration

Fig. 5. Vitamin B12 supplementation in the host altered the transcriptome of P. acnes in the skin microbiota. Vitamin B12 supplementation in the host altered.

A: Photomicrograph of a fibrin gel containing ∼50 islets 2 h after polymerization of a 5-μl fibrinogen solution containing the islets with 5 μl thrombin.

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

Fig. 2 Surface modification, characterization, and verification of Tz-grafted SiNWS. Surface modification, characterization, and verification of Tz-grafted.

Fig. 1 Responsive lock-and-key microgel particle characterization.

Fig. 2 Images of complex structures formed via sequential folding.

The microchip-based drug delivery device and overview of study design

Fig. 5 Chaetopterus tube lining formed in seawater or within sediment.

Fig. 2. LUM015 fluorescently labels tumor cells in mouse models of STS and breast cancer. LUM015 fluorescently labels tumor cells in mouse models of STS.

Fig. 2 PK dosing results. PK dosing results. (A) Plasma concentration of hPTH(1–34) versus time after release of 40-μg dose from implanted microchip device.

oCVD synthesis process, molecular structure, and film morphology

Fig. 3 Library of 8988 macrocycles screened against thrombin.

Fig. 3 Imaging-guided S-HDL nanoimmunotherapy in rabbits and pigs: PET-based readouts. Imaging-guided S-HDL nanoimmunotherapy in rabbits and pigs: PET-based.

by J. L. Guo, Y. S. Kim, V. Y. Xie, B. T. Smith, E. Watson, J. Lam, H

Fig. 6. Microglial phagocytosis and lysosomal uptake of Aβ induced by SUS treatment. Microglial phagocytosis and lysosomal uptake of Aβ induced by SUS.

CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells. CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial.

Fig. 5. Vascularization of human liver seed grafts.

Tissue architecture affects function of expanded liver seed grafts

Fig. 2. Overexpression of miR cluster in the developing heart results in increased cardiomyocyte proliferation and cardiomegaly. Overexpression.

Fig. 6. Confocal image series at 10-μm intervals through the full retinal thickness at P48 in WT and vldlr-null mice. Confocal image series at 10-μm intervals.

Fig. 2 Solution properties of S-PEDOT.

Fig. 2 NP characterization.

Fig. 4. HERV-K env expression and injury to lower motor neurons.

Fig. 2 Investigating the interactions between the n-type polymer and the enzyme, which lead to efficient electrical communication. Investigating the interactions.

Fig. 4. miR promotes cardiomyocyte proliferation through regulation of Hippo pathway kinases. miR promotes cardiomyocyte proliferation through.

miR can promote cardiomyocyte proliferation in the adult heart

Fig. 2 Increasing KLF17, CDH1, and LASS2 expression reduced malignant progression and promoted apoptosis of tumor cells. Increasing KLF17, CDH1, and LASS2.

Fig. 3 Characterization of SGN responses to optogenetic stimulation.

Fig. 1. Construction of human liver seed grafts.

Fig. 8 DMN-Tre detects Mtb in sputum samples from patients with tuberculosis, similar to the auramine stain. DMN-Tre detects Mtb in sputum samples from.

Fig. 4 Single-particle contact angle measurements.

Fig. 2. Col IV–Ac2-26 NPs increase subendothelial collagen in Ldlr−/− mice with established atherosclerosis. Col IV–Ac2-26 NPs increase subendothelial.

Fig. 3. Association between peak CTL019 expansion and response.

Fig. 1 Schematic structure of a fluorescently labeled eGLP1-conjugated MALAT1 ASO and internalization of fluorescent eGLP1 and eGLP1-MALAT1-ASO. Schematic.

Fig. 3. Col IV–Ac2-26 NPs suppress lesional superoxide and increase lesional Il10 mRNA in Ldlr−/− mice with established atherosclerosis. Col IV–Ac2-26.

Fig. 1. Schematic description of whole-exome or targeted next-generation sequencing analyses. Schematic description of whole-exome or targeted next-generation.

Fig. 4 Characterization and SERS spectra of tetrameric metamolecules.

The neuropod cells. The neuropod cells. (Top left) Neuropod cells synapse with sensory neurons in the small intestine, as shown in a confocal microscopy.

Fig. 6. Connectivity and mobility maps for Lesotho.

Presentation transcript:

Fig. 1. PolySTAT synthesis and characterization. PolySTAT synthesis and characterization. (A) The PolySTAT polymer backbone, a linear statistical copolymer of HEMA and NHSMA synthesized via RAFT polymerization, was grafted with the cyclic fibrin-binding peptide Ac-Y(DGl)C(HPr)YGLCYIQGK-Am (19) through NHS ester reaction with the lysine ε-amine. DGl, d-glutamic acid; HPr, hydroxyproline; Ac, acetylated N terminus; Am, amidated C terminus. (B) Two different polymer backbones were used, including a fluorescent FMA-modified p(HEMA-co-NHSMA) for confocal studies. Polymer backbones were grafted with the fibrin-binding peptide for PolySTAT or a nonbinding scrambled peptide for the PolySCRAM control. Polymer molecular weight (MW) and polydispersity index (PDI) were determined using gel permeation chromatography (GPC). Peptides per polymer were calculated using ultraviolet (UV) absorbance. NA, not applicable. (C) PolySTAT integration into fibrin was confirmed using confocal imaging. Pure fibrin clots were made using a solution of Alexa Fluor 546–labeled fibrinogen (red) and thrombin mixed with PBS, fPolySTAT (green), or fPolySCRAM (green). Scale bars, 10 μm. Leslie W. Chan et al., Sci Transl Med 2015;7:277ra29 Copyright © 2015, American Association for the Advancement of Science