Crystal Structure of Entamoeba histolytica Cdc45 Suggests a Conformational Switch that May Regulate DNA Replication Fredy Kurniawan, Ke Shi, Kayo Kurahashi,

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Crystal Structure of Entamoeba histolytica Cdc45 Suggests a Conformational Switch that May Regulate DNA Replication Fredy Kurniawan, Ke Shi, Kayo Kurahashi, Anja-Katrin Bielinsky, Hideki Aihara iScience Volume 3, Pages 102-109 (May 2018) DOI: 10.1016/j.isci.2018.04.011 Copyright © 2018 The Author(s) Terms and Conditions

iScience 2018 3, 102-109DOI: (10.1016/j.isci.2018.04.011) Copyright © 2018 The Author(s) Terms and Conditions

Figure 1 Sequence Alignment between Human and Entamoeba histolytica Cdc45 Secondary structures for E. histolytica Cdc45 are shown schematically above the sequence. η and TT denote 310-helix and β-turn, respectively. The 11-residue stretch (amino acids S154 to I164) deleted to facilitate crystallization of human Cdc45 (Simon et al., 2016) is indicated. This figure was prepared using ESPript3.0 (http://espript.ibcp.fr) (Robert and Gouet, 2014). iScience 2018 3, 102-109DOI: (10.1016/j.isci.2018.04.011) Copyright © 2018 The Author(s) Terms and Conditions

Figure 2 Overall Structure of E. histolytica Cdc45 (A) Ribbon model of E. histolytica Cdc45 viewed from two orthogonal orientations. Color coding for the four structural domains matches that used in Figure 1. Residues 130 and 171 on either end of the disordered region are highlighted by small blue spheres. The disordered residues (131–170) are represented by a dotted line in the right panel. (B) A schematic diagram showing domain organization of E. histolytica Cdc45. iScience 2018 3, 102-109DOI: (10.1016/j.isci.2018.04.011) Copyright © 2018 The Author(s) Terms and Conditions

Figure 3 Structural Comparison (A) E. histolytica Cdc45 structure. (B) Zoomed-in view of the region highlighted by a dotted box in panel A. The loop between α9 and β6 of E. histolytica Cdc45 takes a unique “tucked-in” conformation. (C) Human Cdc45 structure, PDB ID: 5dgo (Simon et al., 2016). (D) E. histolytica Cdc45, with the C-terminal DHHA1 domain highlighted in red. A possible coordination between positioning of the DHHA1 domain, α14 helix, and the Mcm5/GINS-interacting loop is depicted in the right panel. (E) Human Cdc45 with the C-terminal DHHA1 domain highlighted. Note distinct positioning of the DHHA1 domain, bent α14 helix, and untucked Mcm5/GINS-interacting loop. (F) Yeast CMG helicase cryo-EM structure (PDB ID: 3jc6) (Yuan et al., 2016) viewed from the N-terminal face of MCM, showing extensive interactions between Cdc45 (ribbon) and Mcm2/5 and the GINS subunits (surface). (G) A full view of the CMG helicase, with the spatial relationship between helicase and DNA polymerase ɛ shown schematically. The catalytic domain of DNA polymerase ɛ can take alternative positions, one of which is stabilized by interaction with the tip of α6 helix of Cdc45 (Zhou et al., 2017). iScience 2018 3, 102-109DOI: (10.1016/j.isci.2018.04.011) Copyright © 2018 The Author(s) Terms and Conditions