Genomic organization of the fruBKA locus in Escherichia coli K-12 and Pseudomonas putida KT2440 and structure of the Cra binding site. Genomic organization.

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Genomic organization of the fruBKA locus in Escherichia coli K-12 and Pseudomonas putida KT2440 and structure of the Cra binding site. Genomic organization of the fruBKA locus in Escherichia coli K-12 and Pseudomonas putida KT2440 and structure of the Cra binding site. (A) Conserved genomic organization of the fruBKA operon in enterobacteria and in P. putida. This gene cluster encompasses fruB (fructose-specific EI/HPr/EIIA component of the PTS), fruK (1-phosphofructokinase), and fruA (fructose-specific subunit IIC of the PTS). Note that while the cra (i.e., fruR) gene lies far away from the fruBKA gene cluster in E. coli, the corresponding orthologue in strain KT2440 forms part of the same genomic locus but located in a divergent fashion. (B) The canonical E. coli consensus sequence for the binding of Cra to DNA was retrieved from the PRODORIC database (53, 54). Note that this sequence forms an incomplete palindrome in which the left half-site is considerably better conserved than the right half-site (the Cra binding sites of P. putida examined in this work are closer to a perfect palindrome; see below). Max Chavarría et al. mSystems 2016; doi:10.1128/mSystems.00154-16