Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen,

Slides:

Advertisements

Similar presentations

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

Advertisements

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

by Rui Zhang, Jeffrey D. Lifson, and Claire Chougnet

Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells Marc A. Williams,

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response Miriam Wittmann, MD, Mareike Alter, Tanja.

CD4 T-helper cells engineered to produce IL-10 prevent allergen-induced airway hyperreactivity and inflammation Jae-Won Oh, MD, PhD, Christine M. Seroogy,

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation Regina K. Rowe, MD, PhD, David M. Pyle, MD,

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of TH1/TFH signatures Jelle de Wit, PhD, Tineke Jorritsma,

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut Claudia.

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Role of IL-9 in the pathophysiology of allergic diseases

Allergen-induced IgE-dependent gut inflammation in a human PBMC–engrafted murine model of allergy Benno Weigmann, PhD, Nadja Schughart, MSc, Christian.

Estrogen enhances immunoglobulin production by human PBMCs

Inhibition of differentiation, amplification, and function of human TH17 cells by intravenous immunoglobulin Mohan S. Maddur, DVM, Janakiraman Vani,

Activated glycoprotein A repetitions predominant (GARP)–expressing regulatory T cells inhibit allergen-induced intestinal inflammation in humanized mice

Volume 141, Issue 3, Pages (September 2011)

Histamine in the immune regulation of allergic inflammation

Human IL-31 is induced by IL-4 and promotes TH2-driven inflammation

Clara cell 16-kd protein downregulates TH2 differentiation of human naive neonatal T cells Sofi Johansson, MSc, Göran Wennergren, MD, PhD, Nils Åberg,

Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,

Norito Katoh, Fujiko Soga, Takeshi Nara, Koji Masuda, Saburo Kishimoto

Requirements for allergen-induced airway inflammation and hyperreactivity in CD4- deficient and CD4-sufficient HLA-DQ transgenic mice Svetlana P. Chapoval,

Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen- presenting cells that induce proallergic T cells Susanne Ebner, PhD,

Inhibition of human B-cell development into plasmablasts by histone deacetylase inhibitor valproic acid Anne-Kathrin Kienzler, MSc, Marta Rizzi, MD,

Regulation of allergic airway inflammation by class I–restricted allergen presentation and CD8 T-cell infiltration James W. Wells, PhD, Christopher J.

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Poly(I:C)-Treated Human Langerhans Cells Promote the Differentiation of CD4+ T Cells Producing IFN-γ and IL-10 Laetitia Furio, Hermine Billard, Jenny.

Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses Stephan Traidl, Petra Kienlin,

Yanli Ouyang, MD, PhDa, Nisha Virasch, BSa, Ping Hao, MDa, Michael T

Thymic stromal lymphopoietin–activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis Wen Hao Wu, PhD,

Peanut-specific type 1 regulatory T cells induced in vitro from allergic subjects are functionally impaired Laurence Pellerin, PhD, Jennifer Anne Jenks,

Role of B cells in TH cell responses in a mouse model of asthma

Mammalian target of rapamycin inhibition counterbalances the inflammatory status of immune cells in patients with chronic granulomatous disease Aurélie.

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Ulrich Zügel, Khusru Asadullah Journal of Investigative Dermatology

Chen Yao, BS, Sandra M. Zurawski, PhD, Elizabeth S

The mannose receptor negatively modulates the Toll-like receptor 4–aryl hydrocarbon receptor–indoleamine 2,3-dioxygenase axis in dendritic cells affecting.

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis Margarete Niebuhr, MD, Helena Scharonow, MS, Merle Gathmann,

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

IFN-τ inhibits IgE production in a murine model of allergy and in an IgE-producing human myeloma cell line Mustafa G. Mujtaba, PhDa, Lorelie Villarete,

Human CD4+ T Lymphocytes with Remarkable Regulatory Functions on Dendritic Cells and Nickel-Specific Th1 Immune Responses Andrea Cavani, Francesca Nasorri,

Staphylococcal enterotoxin A–activated regulatory T cells promote allergen-specific TH2 response to intratracheal allergen inoculation Wei-ping Zeng,

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Ganglioside GQ1b enhances Ig production by human PBMCs

Modification of the human allergic immune response by allergen-DNA–transfected dendritic cells in vitro Bettina Klostermann, MSc, Iris Bellinghausen,

Double-Stranded RNA-Exposed Human Keratinocytes Promote Th1 Responses by Inducing a Type-1 Polarized Phenotype in Dendritic Cells: Role of Keratinocyte-Derived.

Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity Iris Bellinghausen, PhD, Sebastian.

Antigen-specific and nonspecific determinants of cytokine production during topical sensitization of mice to chemical allergens Artin Moussavi, PhDa,

Fetal and neonatal IL-13 production during pregnancy and at birth and subsequent development of atopic symptoms Toby J. Williams, Catherine A. Jones,

David M. Essayan, MD, Charity C

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Expression and function of histamine receptors in human monocyte-derived dendritic cells Marco Idzko, MDa, Andrea la Sala, PhDb, Davide Ferrari, PhDc,

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Duy Pham, PhD, Sarita Sehra, PhD, Xin Sun, PhD, Mark H. Kaplan, PhD

Qianli Zhuang, MD, PhD, Bruce Mazer, MD

The mannose receptor negatively modulates the Toll-like receptor 4–aryl hydrocarbon receptor–indoleamine 2,3-dioxygenase axis in dendritic cells affecting.

In vivo effects of glucocorticoids on IgE production

Lactic acid bacteria inhibit TH2 cytokine production by mononuclear cells from allergic patients Pierre Pochard, PhDab, Philippe Gosset, PhDb, Corinne.

IL-10–producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients Antje Prasse, MD, Martin Germann,

Differences in antigen-specific T-cell responses between infants with atopic dermatitis with and without cow’s milk allergy: Relevance of TH2 cytokines

The novel synthetic immune response modifier R-848 (Resiquimod) shifts human allergen-specific CD4+ TH2 lymphocytes into IFN-γ–producing cells Francesca.

Neuropeptide signaling through neurokinin-1 and neurokinin-2 receptors augments antigen presentation by human dendritic cells Junya Ohtake, PhD, Shun.

Perturbations of natural killer cell regulatory functions in respiratory allergic diseases Francesca Scordamaglia, MD, Mirna Balsamo, PhD, Antonio Scordamaglia,

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites

Presentation transcript:

Inhibition of human allergic T-cell responses by IL-10–treated dendritic cells: Differences from hydrocortisone-treated dendritic cells Iris Bellinghausen, PhD, Udo Brand, PhD, Kerstin Steinbrink, MD, Alexander H. Enk, MD, Jürgen Knop, MD, Joachim Saloga, MD Journal of Allergy and Clinical Immunology Volume 108, Issue 2, Pages 242-249 (August 2001) DOI: 10.1067/mai.2001.117177 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Modulation of surface marker expression of allergen-pulsed DCs by IL-10 and HC. DCs were matured without (control ) or with the addition of IL-10 or HC on day 7 of culture. After 48 hours, cells were double-stained for HLA-DR and additional marker, as indicated. The figure shows 1 representative example, including the mean fluorescence intensity and the percentage of positive cells (for CD83), of 15 separate experiments. Journal of Allergy and Clinical Immunology 2001 108, 242-249DOI: (10.1067/mai.2001.117177) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 Inhibition of cytokine production of allergen-pulsed mature DCs by treatment with IL-10 and HC. DCs were matured without (control ) or with the addition of IL-10 or HC on day 7 of culture. Supernatants were harvested 24 hours later for assessment of IL-12p40 and IL-12p70 (n = 11) production and after 48 hours for analysis of IL-6 (n = 11) and IL-10 (n = 9) production by ELISA. The results represent the means ± SDs. Asterisks indicate statistically significant differences (P ≤ .05) between untreated and IL-10– or HC-treated DCs. n.s., Not shown. Journal of Allergy and Clinical Immunology 2001 108, 242-249DOI: (10.1067/mai.2001.117177) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Inhibition of the T cell–stimulatory capacity of allergen-pulsed DCs by IL-10 and HC. 1 × 105 CD45RA+ or CD45R0+ CD4+ T cells were stimulated with titrated numbers of autologous allergen-pulsed DCs, either pretreated with IL-10 or HC or else untreated. After 5 days, the cells were pulsed with 1 μCi of [3H]TdR for 6 hours before harvesting. The data represent the means ± SDs from 8 independent experiments. Asterisks indicate statistically significant differences (P ≤ .05) between untreated and IL-10– or HC-treated DCs. Journal of Allergy and Clinical Immunology 2001 108, 242-249DOI: (10.1067/mai.2001.117177) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 4 IL-10– or HC-treated DCs differentially modulate cytokine production of naive and memory T helper cells from atopic donors. 5 × 105 CD45RA+ or CD45R0+ CD4+ T cells were stimulated with 5 × 104 autologous allergen-pulsed DCs, either pretreated with IL-10 or HC or else untreated. After 1 week of culture, T cells were restimulated with 5 × 104 newly generated DCs or with IL-10– or HC-treated DCs of the same donor. Supernatants were collected 24 hours later and analyzed for IL-4, IL-5, and IFN-γ content by ELISA. Shown are the means ± SDs from 19 (for IL-10–treated DCs) and 16 (for HC-treated DCs) independent experiments. Asterisks indicate statistically significant differences (P ≤ .05) between untreated and IL-10– or HC-treated DCs. Journal of Allergy and Clinical Immunology 2001 108, 242-249DOI: (10.1067/mai.2001.117177) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 5 Long-lasting and stable effect of IL-10– or HC-pretreatment of DCs on cytokine production by T cells. 5 × 105 CD45RA+ or CD45R0+ CD4+ T cells were primarily stimulated with 5 × 104 autologous allergen-pulsed DCs, either pretreated with IL-10 or HC or else untreated. After 1 week of culture, T cells were restimulated with 5 × 104 newly generated, fully mature, allergen-pulsed DCs of the same donor. Supernatants were collected 24 hours later and analyzed for IL-4, IL-5, and IFN-γ content by ELISA. Shown are the means ± SDs from 26 (for IL-10–treated DCs) and 24 (for HC-treated DCs) independent experiments. Asterisks indicate statistically significant differences (P ≤ .05) between untreated and IL-10– or HC-treated DCs. Journal of Allergy and Clinical Immunology 2001 108, 242-249DOI: (10.1067/mai.2001.117177) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 6 IL-2 restores the cytokine response to cells previously inhibited by coculture with IL-10– or HC-treated DCs. 5 × 105 CD45RA+ CD4+ T cells were primarily stimulated with 5 × 104 autologous allergen-pulsed DCs, either pretreated with IL-10 or HC or else untreated. After 1 week of culture, T cells were restimulated with 5 × 104 newly generated, mature, allergen-pulsed DCs of the same donor without or with the addition of 100 U/mL IL-2. Supernatants were collected 24 hours later and analyzed for IL-4, IL-5, and IFN-γ content by ELISA. Shown are the means ± SDs from 8 independent experiments. Journal of Allergy and Clinical Immunology 2001 108, 242-249DOI: (10.1067/mai.2001.117177) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 7 IL-10– and HC-pretreated DCs differentially modulate allergen-specific T-cell responses. Whereas pretreatment of allergen-pulsed DCs with IL-10 induces functional hyporesponsiveness of allergen-specific TH1 and TH2 cells, HC-pretreated DCs inhibit TH1 but not TH2 cells. Journal of Allergy and Clinical Immunology 2001 108, 242-249DOI: (10.1067/mai.2001.117177) Copyright © 2001 Mosby, Inc. Terms and Conditions