TH2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis Shin Morizane, MD, PhD, Kenshi Yamasaki, MD, PhD, Ai Kajita,

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Presentation transcript:

TH2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis Shin Morizane, MD, PhD, Kenshi Yamasaki, MD, PhD, Ai Kajita, MD, Kazuko Ikeda, MD, Maosheng Zhan, MD, Yumi Aoyama, MD, PhD, Richard L. Gallo, MD, PhD, Keiji Iwatsuki, MD, PhD Journal of Allergy and Clinical Immunology Volume 130, Issue 1, Pages 259-261.e1 (July 2012) DOI: 10.1016/j.jaci.2012.03.006 Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, NHEKs were treated with inflammatory cytokines, and levels of KLK7 protein in the cultured media were measured by using ELISAs. B-I, NHEKs were stimulated with IL-4 (50 ng/mL) or IL-13 (50 ng/mL), and the relative mRNA abundance of KLK7 (KLK7), KLK5 (KLK5), KLK8 (KLK8), LEKTI (SPINK5), elafin (PI3), secretory leukocyte protease inhibitor (SLPI), filaggrin (FLG), and loricrin (LOR) was analyzed by using real-time PCR. *P < .05 and **P < .01. Data are means ± SEMs of triplicate samples and representative of 3 independent experiments. Journal of Allergy and Clinical Immunology 2012 130, 259-261.e1DOI: (10.1016/j.jaci.2012.03.006) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 A and B, NHEKs were stimulated with IL-4 (50 ng/mL) or IL-13 (50 ng/mL), and chymotrypsin-like (Fig 2, A) or trypsin-like (Fig 2, B) serine protease activity was measured by using the specific substrates. *P < .05 and ***P < .001. Data are means ± SEMs of triplicate samples and representative of 3 independent experiments. C, KLK7 mRNA expression was analyzed by using real-time PCR and normalized with the mean of normal skin. *P < .05. D, KLK7 protein expression was examined by using immunohistochemistry. Bar = 100 μm. E, KLK7 and IL-4 levels in the sera of patients with AD were measured by using ELISAs and the correlation was analyzed with the Spearman rank correlation coefficient. Journal of Allergy and Clinical Immunology 2012 130, 259-261.e1DOI: (10.1016/j.jaci.2012.03.006) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions