Ajay B. Antony, HSa, Robert S. Tepper, MDb, Kamal A. Mohammed, PhDa 

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Presentation transcript:

Cockroach extract antigen increases bronchial airway epithelial permeability  Ajay B. Antony, HSa, Robert S. Tepper, MDb, Kamal A. Mohammed, PhDa  Journal of Allergy and Clinical Immunology  Volume 110, Issue 4, Pages 589-595 (October 2002) DOI: 10.1067/mai.2002.127798 Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 1 CrAg-induced VEGF release in BAECs: A , concentration response curve; B , time-dependent response to 32 μg/mL CrAg. Data represent mean ± SE of 3 separate experiments. Asterisk denotes statistical significance (P < .001) in comparison with control cultures. Journal of Allergy and Clinical Immunology 2002 110, 589-595DOI: (10.1067/mai.2002.127798) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 2 CrAg-induced expression of VEGF family members in BAECs: A , resting BAECs; B , CrAg-induced BAECs. Lanes 1 , 2 , 3 , and 4 represent VEGFA -, VEGFB -, VEGFC -, and VEGFD -specific genes, respectively. GAPDH served as a positive control gene. Results are representative of 3 independent experiments. Journal of Allergy and Clinical Immunology 2002 110, 589-595DOI: (10.1067/mai.2002.127798) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 3 CrAg-induced VEGFA mRNA expression in BAECs: A , resting BAECs; B , CrAg-stimulated BAECs. Lane M indicates molecular weight marker (Hae-III), and lanes 1-4 indicate 3, 6, 12, and 24 hours, respectively. GAPDH served as a positive housekeeping gene. Results are representative of 3 independent experiments. Journal of Allergy and Clinical Immunology 2002 110, 589-595DOI: (10.1067/mai.2002.127798) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 4 Real-time electrical resistance across BAEC monolayer. Values represent mean ± SE of 3 separate experiments. Asterisks denote statistical significance (P < .001) in comparison with control (unstimulated) cultures. Journal of Allergy and Clinical Immunology 2002 110, 589-595DOI: (10.1067/mai.2002.127798) Copyright © 2002 Mosby, Inc. Terms and Conditions