BRAF Mutations Are Common Somatic Events in Melanocytic Nevi1

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BRAF Mutations Are Common Somatic Events in Melanocytic Nevi1 Rajiv Kumar, Sabrina Angelini, Erna Snellman, Kari Hemminki Journal of Investigative Dermatology Volume 122, Issue 2, Pages 342-348 (February 2004) DOI: 10.1046/j.0022-202X.2004.22225.x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Detection of BRAF mutations in melanocytic nevi (B) An autoradiograph showing SSCP analysis for the detection of T1799A (V600E) mutation in exon 15 of the BRAF gene. Aberrant band shifts in lanes 2, 4, and 6 are due to the mutations in DNA from nevi from cases 16 and 17. Lane 1, migration pattern of wild-type single strands of amplified DNA from the surrounding tissue corresponding to one nevus from case 16; lane 2, aberrant band owing to mutation in second nevus from case 16; lane 3, amplified DNA from corresponding surrounding tissue; lane 4, DNA from one nevus from case 17 with mutation; lane 5, corresponding surrounding tissue; lane 6, DNA from second nevus from case 17; lane 7, DNA from corresponding surrounding tissues; lanes 8 and 9, no template controls; and lane 10, a control DNA. (A) An autoradiograph showing heteroduplex analysis of exon 15 fragment of the BRAF gene in nevi and corresponding surrounding tissues. The double-strand DNA in lane 6 shows the aberrant band shift owing to A1781G (D594V) mutation in the BRAF gene in one nevus from case 8. This mutation did not cause any shift in the single strands. Lane 7, DNA from corresponding surrounding tissue. (D) Sequence analysis showing T1799A mutation (arrow) in exon 15 of the BRAF gene in nevus from case 16. (C) Part of sequence showing A1781G mutation (arrow) in exon 15 of the BRAF gene in nevus from case 8. (E) Part of sequence of exon 15 of the BRAF gene obtained from a control DNA (from lymphocytes of a healthy individual) corresponding to wild-type sequence reported in gene data bank (accession No. NT_007914). Arrows, the positions of mutations in nevi from cases 8 and 16. Journal of Investigative Dermatology 2004 122, 342-348DOI: (10.1046/j.0022-202X.2004.22225.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions