C. elegans phenotypes created by H1. X RNA interference

Slides:

Advertisements

Similar presentations

Mitochondria localize to both the front and the rear of neutrophils.

Advertisements

Identification of vegetally localised vrtn transcripts.

Chronic stress accelerated DSS-induced colitis.

Volume 106, Issue 1, Pages (July 2001)

Fig. 1 HIV transcription profiles in freshly isolated CD4+ T cells and PBMCs from HIV-infected patients on ART suggest blocks to HIV transcriptional elongation,

Ezequiel Alvarez-Saavedra, H. Robert Horvitz Current Biology

CK2 is required for early cell divisions in C. elegans embryos

Differential secretion profiles of C. elegans TTR models.

Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle

No significant functional difference could be observed between N1ICD and N2ICD in maintaining skin barrier function and promoting tumorigenesis. No significant.

Fig. 3. Knockdown of cited3 results in increased cell death but it does not affect proliferation.Embryos that are injected with the control MO (A–C), cited3.

Developmental defects in the svkA-null mutant.

PARP cleavage is detected in TA cells, but not in KSC

IQGAPs localize at the tips of axons in hippocampal neurons.

RNAi-induced loss of Ce-emerin protein persists into adulthood.

Bgs1p does not localize to the contractile ring during or after mitosis in the septation initiation network mutants cdc and cdc Bgs1p does.

Fig. 6. LR phenotype of plastid translation-defective mutants with/without Spec. LR phenotype of plastid translation-defective mutants with/without Spec.

NFAT5+/- muscle cells are less motile.

Caspase 8 is expressed in human ESCs but is not regulated by IFN-γ, TNF-α or hCG. Caspase 8 is expressed in human ESCs but is not regulated by IFN-γ, TNF-α.

Western blot analysis of histone H1.X.

The expression of histone H1. X::GFP in C

Barc knockdown causes intron retention of short, GC-rich introns with weak splice sites. barc knockdown causes intron retention of short, GC-rich introns.

Fig. 7. Lhx1-RNAi reduces the eye size

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

H1. X depletion by RNA interference and colocalization of H1

Loss of serotonergic markers after Smed-pitx RNAi is not due to stem cell-based turnover. Loss of serotonergic markers after Smed-pitx RNAi is not due.

(A) Counterclockwise contour Γ around a crack tip showing an element of path length ds, with unit vector n normal to the path and with stress and displacement.

Homozygous col22a1−/− embryos show increased percentage of hemorrhages when raised at increased temperature. Homozygous col22a1−/− embryos show increased.

Characterization of anti-N-mPres.

Effect of Dipy on the expression of chondrogenic markers.

Maintained Pax7 expression delays the onset of myogenin expression.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

(A) Diagram illustrating axes of mitoses at different stages of follicle development and in different layers of the GCs. Thicker lines mark predominant.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Functions of AP-1 subunits in various cellular processes and disease.

The expression of two forms of N-cadherin.

Streaming phenotypes are developmentally regulated.

E4-ORF1 disrupts proper TJ localization of ZO-1 and ZO-2 in MDCK cells

FRAP and FLIP analysis of GFP-ADAR2 and GFP-ADAR1C-Term.

Ectopic Pax7 increases MyoD expression in Pax7-null myogenic cells.

EB1 localization to the centrosome is mediated by its C-terminal domain. EB1 localization to the centrosome is mediated by its C-terminal domain. Sections.

Peroxisome speeds were slower in patient and control cells.

EB1 and its C-terminal binding partner APC co-purify with centrosomes.

Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

mip120 null egg chambers have a condensed nurse cell DNA phenotype

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

Integrin-mediated signaling is required for PFC maturation.

Integrins are required to strengthen Notch signaling.

Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted Drosophila S2 cells. Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted.

Integrins modulate the Notch pathway by regulating its intracellular trafficking and/or processing. Integrins modulate the Notch pathway by regulating.

Fig. 7. Rho family GTPase signaling is required for cyst formation in Ptp4E Ptp10D mutants.(A–C) Expression of DN Rho1 and Rac1 mutants in wild-type (w1118)

FAK is required for LPA-mediated induction of trailing-edge retraction and the restoration of normal morphology. FAK is required for LPA-mediated induction.

The stenotele explosion process (modified from Tardent and Holstein, 1982). The stenotele explosion process (modified from Tardent and Holstein, 1982).

(A,B) Immunofluorescence of Cx43 (red) and smooth muscle actin (green) in cultured myometrial cells from untreated (A) and tamoxifen-treated (B) Cx43fl/fl:SM-CreERT2.

Model of local recycling of the actin network in L

SH3 domain of spectrin accumulates in the early integrin clusters.

Subnuclear localization of HA-tagged EBNA1 protein.

Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM,

Ezrin phenotypes in the MVID intestine.

Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. Tsg protein stimulates endothelial cell migration,

Large pericentromeres colocalize more frequently with H3S10P foci than small pericentromeres. Large pericentromeres colocalize more frequently with H3S10P.

Distinct mitochondrial membrane potential, ATP-to-ADP ratio and NAD(P)H responses to glucose and methylsuccinate. Distinct mitochondrial membrane potential,

Phenotype of S. pombe cells in the presence of B21P2 or LMB

Phylogenetic analysis and domain structure of CALM proteins.

Flow field adjacent to an eel (body length L=0

4E-BP is present in an 80 kDa complex in unfertilized eggs.

Estimating statistical significance using the overlap rule for 95% CI bars. Estimating statistical significance using the overlap rule for 95% CI bars.

BR Biological Activity of DS

Presentation transcript:

C. elegans phenotypes created by H1. X RNA interference C. elegans phenotypes created by H1.X RNA interference. 61 animals from 1351 scored F1 animals (4.5%) are small and dumpy (A). C. elegans phenotypes created by H1.X RNA interference. 61 animals from 1351 scored F1 animals (4.5%) are small and dumpy (A). They reach only half of the final body length of normal C. elegans. Half of these animals (34 of 61) are additionally defective in egg laying (B). Another phenotype affected 3% of the F1 population (33 animals of 1351). These animals contain an abnormally elongated pharynx (C). A control animal is shown in (D; the arrows point to the pharynx). Bar, 20 μm. Monika A. Jedrusik et al. J Cell Sci 2002;115:2881-2891 © The Company of Biologists Limited 2002