An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic progenitor cells. An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic.

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An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic progenitor cells. An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic progenitor cells. (A) Micrographs showing the result of immunostaining for HNF4A in iPSC-derived hepatic progenitor cells treated between days 6 and 8 of differentiation with either DMSO (n=6 wells) or the HSP90β inhibitor CCT-018159 (n=6 wells). Scale bar: 100 µm. (B) Bar graph showing the quantification of HNF4A levels by measuring the average pixel intensity using ImageJ. Significance was determined by Student's unpaired t-test (mean±s.e.m., P≤0.001, n=6). (C) Representative western blot used to measure the steady-state levels of intracellular HNF4A in iPSC-derived hepatic progenitor cells treated with either DMSO (control) or CCT-018159 between days 6 and 8 of differentiation. GAPDH was used as a loading control. (D) Micrographs showing immunostaining for HNF4A in hepatic progenitor cells treated with DMSO or 20, 10, 5, 2.5, 1.25, 0.625, 0.312 and 0.156 mM CCT-018159. DAPI staining was used as a indication of cell viability. Scale bar: 100 µm. (E) Graph showing the CCT-018159 dose-response curve, determined by quantification of HNF4A immunostaining by ImageJ, that was used to calculate the IC50 (http://ic50.tk) (n=3). Ran Jing et al. Development 2017;144:1764-1774 © 2017. Published by The Company of Biologists Ltd