Fig. 4. SMXL6 is degraded in response to SL treatment.

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Fig. 4. SMXL6 is degraded in response to SL treatment. SMXL6 is degraded in response to SL treatment. (A) Expression of SMXL6-YFP in vascular cambium cells of max2-1 stems (yellow). Purple signal indicates chloroplast autofluorescence. (B-D) Response of SMXL6-YFP protein levels in Col-0 roots to treatment with 5 µM rac-GR24 over a 10 min time course. (E-H) Comparison of SMXL6-YFP protein levels in Col-0 roots after 20 min treatment with solvent control (E) 5 µM KAR1 (G) or 5 µM rac-GR24 in the presence (H) or absence (F) of MG132, an inhibitor of the 26S proteasome. (I,J) Comparison of SMXL6 protein levels in max2-1 roots after 20 min treatment with solvent control (I) or 5 µM rac-GR24 (J). (K,L) Comparison of SMXL6Δpl-YFP protein levels in roots after 20 min treatment with solvent control (K) or 5 µM rac-GR24 (L). Tom Bennett et al. Biology Open 2016;5:1806-1820 © 2016. Published by The Company of Biologists Ltd