FAK/Src activity is required to trap PTP1BDA in adhesions.

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FAK/Src activity is required to trap PTP1BDA in adhesions. FAK/Src activity is required to trap PTP1BDA in adhesions. (A,B) FAK KO cells were transfected with mRFP-PTP1BDA and reconstituted with (A) GFP–FAK or (B) GFP–FAK-Y397F. Colors were inverted for better visualization. (C–H) SYF cells co-transfected with GFP-PTP1BDA and Src (C), Src KD (D), Src Y529F (E), Src KD/Y529F (F), Src Y418F (G) or Src Y418F/Y529F (H). In all these cells vinculin was detected by immunofluorescence. GFP–PTP1BDA puncta associated with peripheral vinculin adhesions (in red) are indicated by white arrowheads in the insets (2× magnification). (I) Plot shows percentages of peripheral adhesions containing GFP–PTP1B puncta. Statistical significance was determined using one-way ANOVA followed by the Tukey's HSD post-hoc test (a,bP<0.0001; c,d; c,eP<0.0001; c,fP = 0.001). Scale bar: 25 µm. Juan E. Burdisso et al. J Cell Sci 2013;126:1820-1831 © 2013. Published by The Company of Biologists Ltd