Evaluation of steroid receptors, coregulators, and molecules associated with uterine receptivity in secretory endometria from untreated women with polycystic.

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Presentation transcript:

Evaluation of steroid receptors, coregulators, and molecules associated with uterine receptivity in secretory endometria from untreated women with polycystic ovary syndrome Susana Quezada, M.Sc., Carla Avellaira, M.Sc., Maria Cecilia Johnson, Ph.D., Fernando Gabler, M.D., Ariel Fuentes, M.D., Margarita Vega, Ph.D. Fertility and Sterility Volume 85, Issue 4, Pages 1017-1026 (April 2006) DOI: 10.1016/j.fertnstert.2005.09.053 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 The PCR amplification from cDNA of the endometria of normal women (NE) and women with PCOS (PCOSE) using primers for ERα (271 bp), ERβ (258 bp), AR (545 bp), PR (400 bp), and GAPDH (195 bp). The normalized yield for ERα, ERβ, PR, and AR PCR fragments relative to GAPDH PCR products from different endometria is presented. Values are mean ± SEM of independent experiments for NE (n = 8) and PCOSE (n = 8). aP<.05 between NE and PCOSE; bP<.001 between NE and PCOSE. Quezada. Characterization of endometria of PCOS-women. Fertil Steril 2006. Fertility and Sterility 2006 85, 1017-1026DOI: (10.1016/j.fertnstert.2005.09.053) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Immunohistochemical detection of estrogen receptor α (ERα), estrogen receptor β (ERβ), progesterone receptor (PR), and androgen receptor (AR) in paraffin wax sections of endometria obtained from normal women (NE) (left panel) and women with PCOS (PCOSE) (right panel). Positive nuclear staining was detected in epithelial cells for ERα (A, B) and for ERβ (C, D) in the nuclei and cytoplasm of epithelial and stromal cells. Nuclear and cytoplasmic staining for PR (E, F) was detected in stroma and epithelia of both groups of endometria, with a higher number of positive cells in the stroma than in epithelia of NE. Positive nuclear and cytoplasmic staining was detected in stroma and epithelia for AR (G, H). Negative control (nonimmune rabbit serum) is used in place of the respective antibody (I). Arrowheads indicate positive staining of the respective proteins. Magnification in all panels is ×400. Scale bars represent 30 μm. Quezada. Characterization of endometria of PCOS-women. Fertil Steril 2006. Fertility and Sterility 2006 85, 1017-1026DOI: (10.1016/j.fertnstert.2005.09.053) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Immunohistochemical localization of steroid receptor coactivators AIB1/SRC-3 and ARA70 and the corepressor NCoR, and the mucin MUC-1 and β3-integrin subunit in paraffin wax sections of normal women (NE) (left panel) and women with PCOS (PCOSE) (right panel). Nuclear and cytoplasmic positive staining for AIB1/SRC-3 (A, B) was detected in NE and PCOSE, with an intense staining in the epithelia of both groups of endometria. The steroid receptor coactivator ARA70 (C, D) was detected predominantly in the cytoplasmic ofepithelial and stromal cells of both NE and PCOSE. The positive staining was visibly increased in epithelia of PCOSE. The immunostaining for the corepressor NCoR (E, F) was detected preferentially in the cytoplasm of epithelia and stroma of both types of endometria, and no differences were observed in the intensity of the staining between NE and PCOSE. Immunihistochemical staining for β3-integrin subunit (G, H) was detected in the cytoplasm of epithelial cells of NE and PCOSE, being markedly lower in PCOSE epithelia. The antiadhesion molecule MUC-1 (I, J) was immunolocalized in the epithelial compartment of both groups of endometria, with anintense positive staining in the cytoplasm of epithelial cells. Negative control (nonimmune rabbit serum) is used in place of the respective antibody (K). Arrowheads indicate positive staining of the respective proteins. Magnification in all panels is ×400. Scale bars represent 30 μm. Quezada. Characterization of endometria of PCOS-women. Fertil Steril 2006. Fertility and Sterility 2006 85, 1017-1026DOI: (10.1016/j.fertnstert.2005.09.053) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions