Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation Hiroshi Tachimoto, MD, PhD, Sherry A. Hudson, MSB, Bruce.

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Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation Hiroshi Tachimoto, MD, PhD, Sherry A. Hudson, MSB, Bruce S. Bochner, MD Journal of Allergy and Clinical Immunology Volume 107, Issue 2, Pages 302-309 (February 2001) DOI: 10.1067/mai.2001.111930 Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 1 Light scatter characteristics (A) and FcεRI α-chain expression (B) of cultured cells. A, Data are shown from a single batch of CHMCs representative of 3 experiments. B, Data are averaged from 3 to 5 separate experiments (mean ± SEM). MFI, Mean fluorescence intensity. Journal of Allergy and Clinical Immunology 2001 107, 302-309DOI: (10.1067/mai.2001.111930) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 2 Expression of β2 integrins during mast cell differentiation. A, Expression of CD18 and CD11a. B, Expression of CD11b and CD11c. Data are mean ± SEM of 3 to 5 separate experiments. MFI, Mean fluorescence intensity. Journal of Allergy and Clinical Immunology 2001 107, 302-309DOI: (10.1067/mai.2001.111930) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 3 Expression of β1 integrins during mast cell differentiation. Expression of CD29 (A), CD49d and CD49e (B), and CD49c and CD49b (C) was examined. Data are mean ± SEM of 3 to 5 separate experiments. Journal of Allergy and Clinical Immunology 2001 107, 302-309DOI: (10.1067/mai.2001.111930) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 4 Expression of β3 integrins and ICAM-1 during mast cell differentiation. Expression of CD51, CD61, and CD54 (ICAM-1) was examined as indicated. Data are mean ± SEM of 3 to 5 (CD51 and CD54) or 1 to 4 (CD61) separate experiments. Journal of Allergy and Clinical Immunology 2001 107, 302-309DOI: (10.1067/mai.2001.111930) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 5 Representative flow cytometric histograms for various adhesion molecules on 1-week-old (gray line) and 14-week-old (black filled) cultured cells. In the upper left panel is labeling with the IgG control mAb, shown for comparison. Journal of Allergy and Clinical Immunology 2001 107, 302-309DOI: (10.1067/mai.2001.111930) Copyright © 2001 Mosby, Inc. Terms and Conditions

Fig. 6 Adhesion of CHMCs to extracellular matrix proteins. A, Fourteen-week-old CHMCs were incubated on the extracellular matrix protein–coated wells in the presence (black bar) or absence (white bar) of 100 ng/mL SCF for 1 hour, and the percentage of adhesion was determined. B, Fourteen-week-old CHMCs were preincubated with the indicated mAb for 30 minutes before the adhesion assay. Data are mean ± SEM of 3 separate experiments. *P < .05. LM, Laminin; VN, vitronectin. Journal of Allergy and Clinical Immunology 2001 107, 302-309DOI: (10.1067/mai.2001.111930) Copyright © 2001 Mosby, Inc. Terms and Conditions