Derivation of developmentally competent oocytes by in vitro culture of preantral follicles retrieved from aged mice Jung Kyu Choi, B.Sc., Jong Il Ahn,

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Presentation transcript:

Derivation of developmentally competent oocytes by in vitro culture of preantral follicles retrieved from aged mice Jung Kyu Choi, B.Sc., Jong Il Ahn, M.S., Jong Heum Park, Ph.D., Jeong Mook Lim, D.V.M., Ph.D. Fertility and Sterility Volume 95, Issue 4, Pages 1487-1489 (March 2011) DOI: 10.1016/j.fertnstert.2010.12.062 Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Reproductive profile and outcome of a combined program of follicle culture and IVF using ovarian follicles from adult (8–11 weeks old) and aged (63–67 weeks old) C57BL/6 mice and the morphology of the follicles and oocytes during in vitro culture. (A) Reproductive profile and outcome of assisted reproductive technology (ART) (B) Morphology of growing follicles and developing oocytes derived from in vitro cultured follicles: (a, e) pseudoantrum formation during follicle culture; (b, f) mature oocytes after follicle culture; (c, g) 2-cell embryo; (d) blastocyst; (h) 8-cell embryo. No significant differences in morphology were detected between oocytes derived from the follicles retrieved from aged and those retrieved from adult mice. Scale bars = 60 μm. Fertility and Sterility 2011 95, 1487-1489DOI: (10.1016/j.fertnstert.2010.12.062) Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions