Tissue inhibitor of metalloproteinase-1 is increased in the saphenofemoral junction of patients with varices in the leg Jose R. Parra, MD, Robert A.

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Presentation transcript:

Tissue inhibitor of metalloproteinase-1 is increased in the saphenofemoral junction of patients with varices in the leg Jose R. Parra, MD, Robert A. Cambria, MD, Chris D. Hower, MD, Michael S. Dassow, BS, Julie A. Freischlag, MD, Gary R. Seabrook, MD, Jonathan B. Towne, MD Journal of Vascular Surgery Volume 28, Issue 4, Pages 669-675 (October 1998) DOI: 10.1016/S0741-5214(98)70093-X Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Representative immunoblot using primary antibody to MMP-2. Lane A, 20 μg of conditioned media from HT-1080 cells (positive control). Lane B, 20 μg of protein homogenate from patient without varices. Lane C, 20 μg of protein homogenate from patient with varicosities. Signal noted at approximately 66 kDa is consistent with MMP-2 and was decreased in the samples from patients with varicose disease. Journal of Vascular Surgery 1998 28, 669-675DOI: (10.1016/S0741-5214(98)70093-X) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Representative immunoblot using primary antibody to TIMP-1. Lane A, 20 μg of conditioned media from HT-1080 cells (positive control). Lane B, 20 μg of protein homogenate from patient without varices. Lane C, 20 μg of protein homogenate from patient with varicosities. Signal noted at approximately 29 kDa is consistent with TIMP-1 and was increased in samples from patients with varicose disease. Journal of Vascular Surgery 1998 28, 669-675DOI: (10.1016/S0741-5214(98)70093-X) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Photomicrographs and immunohistochemistry (magnification, 100×) of vein sections. L, Region of the lumen. A, Trichrome-stained section of saphenous vein near the groin from patient without varices. The media is composed entirely of well-organized smooth muscle cells. B, Trichrome-stained section of saphenous vein near the groin from patient with varices located lower in the leg. The media is infiltrated with collagen, and the smooth muscle architecture is disrupted. C, Immunohistochemical stain using monoclonal antibody for MMP-9 in section from patient without varices. Positive staining is noted only in adventitial and perivascular region. A paucity of staining is noted in the media. D, Immunohistochemical stain using monoclonal antibody for MMP-9 in section from patient with varices. A similar but less intense pattern of positive staining is noted compared with c despite infiltration of the media with collagen. Similar patterns of staining were noted with MMP-2 and TIMP-1 antibodies (data not shown). Journal of Vascular Surgery 1998 28, 669-675DOI: (10.1016/S0741-5214(98)70093-X) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions