BTG2-mediated hepatic gluconeogenesis is mediated by Nur77.

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BTG2-mediated hepatic gluconeogenesis is mediated by Nur77. BTG2-mediated hepatic gluconeogenesis is mediated by Nur77. A: Mouse primary hepatocytes were infected with Ad-GFP, Ad-Btg2, and Ad-DN-Nur77 at a multiplicity of infection of 60, and then hepatic glucose output was measured using glucose-free media supplemented with the sodium lactate (20 mmol/L) and sodium pyruvate (1 mmol/L). B: qPCR analysis for expression of Creb and hepatic gluconeogenic genes. Total RNAs were prepared from primary hepatocytes under the same conditions. C and D: AML-12 cells were transiently transfected with Btg2, Nur77, Creb, DN-Nur77, Hnf-4α, GR, Pgc-1α, and the indicated reporter genes. *P < 0.05, **P < 0.01 vs. untreated control or individual transfected cells; #P < 0.05 vs. Ad-Btg2. FSK, forskolin. Yong Deuk Kim et al. Diabetes 2014;63:1870-1880 ©2014 by American Diabetes Association