Volume 10, Issue 9, Pages (September 2017)

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Volume 10, Issue 9, Pages 1246-1249 (September 2017) CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing Xianrong Xie, Xingliang Ma, Qinlong Zhu, Dongchang Zeng, Gousi Li, Yao-Guang Liu Molecular Plant Volume 10, Issue 9, Pages 1246-1249 (September 2017) DOI: 10.1016/j.molp.2017.06.004 Copyright © 2017 The Author Terms and Conditions

Figure 1 The CRISPR-GE Toolkit for Genome Editing. (A) The overall workflow of CRISPR-GE. (B) The submission page of targetDesign. (C) The results page of targetDesign and a table of results. Low-efficiency sites with contiguous “T” bases (four or more), very low or very high GC content (≤25% or ≥80%), and contiguous base-pairing with the sgRNA sequence are indicated with “Bad site warning” marks (!, !!, and !!!). The mismatched bases in potential off-target sites are shown in red. (D) The submission page of offTarget. (E) Using primerDesign-V to generate primers for preparation of sgRNA expression cassette(s). (F) The results page of DSDecodeM. Multiple (up to 20) sequencing files (ab1 format) can be uploaded at the same time for decoding. All decoding results are displayed in the page and can be downloaded in a file (.txt) by clicking “here”. Molecular Plant 2017 10, 1246-1249DOI: (10.1016/j.molp.2017.06.004) Copyright © 2017 The Author Terms and Conditions