Regulation of human oviductin mRNA expression in vivo

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Presentation transcript:

Regulation of human oviductin mRNA expression in vivo Christine Briton-Jones, B.Sc., Ingrid Hung Lok, Pong Mo Yuen, Tony Tak Yu Chiu, Lai Ping Cheung, Christopher Haines, M.D.  Fertility and Sterility  Volume 75, Issue 5, Pages 942-946 (May 2001) DOI: 10.1016/S0015-0282(01)01696-X

Figure 1 (A), RT-PCR oviductin product. The 410-bp oviductin band (a). The Sac 1 restriction digest of the oviductin RT-PCR product showing two bands, 235 bp and 174 bp; mw, 50-bp ladder molecular weight marker (b). (B), RT-PCR products for the control gene β-actin (543 bp) and for oviductin (410 bp). mRNA from oviductal cells obtained during the early follicular phase with low circulating concentrations of E2, LH, and P (a). mRNA from oviductal cells obtained during the late follicular phase with high circulating concentrations of E2 and LH and low concentration of P (b). mRNA from oviductal cells obtained during the luteal phase with low circulating concentrations of E2 and LH and high concentrations of P (c). Briton-Jones. Regulation of human oviductin in vivo. Fertil Steril 2001. Fertility and Sterility 2001 75, 942-946DOI: (10.1016/S0015-0282(01)01696-X)

Figure 2 (A), Scatterplot showing the correlation between serum E2 concentration and relative oviductin mRNA expression, excluding samples obtained in the luteal phase, where P > 5 nmol/L. (B), Scatterplot showing the correlation between serum LH concentration and the relative oviductin mRNA expression. (C), Scatterplot showing the correlation between serum P concentration and the relative oviductin mRNA expression. Briton-Jones. Regulation of human oviductin in vivo. Fertil Steril 2001. Fertility and Sterility 2001 75, 942-946DOI: (10.1016/S0015-0282(01)01696-X)