Wnt/β-catenin inhibition delays but does not inhibit T/Bra::GFP expression. Wnt/β-catenin inhibition delays but does not inhibit T/Bra::GFP expression.

Slides:

Advertisements

Similar presentations

GDF5 reduces MMP13 expression in human chondrocytes via DKK1 mediated canonical Wnt signaling inhibition L. Enochson, J. Stenberg, M. Brittberg, A. Lindahl

Advertisements

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Volume 7, Issue 4, Pages (October 2016)

Fig. 7. Vinculin recruitment enhances the efficiency of barrier formation.(A) TER measurements after a calcium switch in α-catenin-depleted MDCK cells.

Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. Retinoic acid sentinels in live embryos and responding.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 2. Proportion of motile objects and track length

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

No significant functional difference could be observed between N1ICD and N2ICD in maintaining skin barrier function and promoting tumorigenesis. No significant.

Fig. 4. A primary screen based on scrape closure

TC-1 silencing sensitized A549 and SPC-A-1 cells to radiation therapy

Wnt/β-catenin signaling regulates FGF signaling during fin regeneration. Wnt/β-catenin signaling regulates FGF signaling during fin regeneration. (A) fgf20a.

Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.

Fig. 5. Onecut transcription factors are important for the correct generation of the mdDA neuronal population.(A) Schematic representation of the region.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. Pigmentation and melanophore counts of rainbow trout parr and smolt caudal fins.Pigmentation of (A) parr and (B) smolt. Pigmentation and melanophore.

Latency to onset of Stage III behavioral seizure activity.

Fig. 2. Two signal-producing behaviours of wild-type Canton-S males and per mutant males relative to whether the wild-type female is moving or immobile.

Fig. 4. Non-autonomous rescue of puc expression in DME cells

Fig. 5. GFP fluorescence colocalization of Gcn5.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 2. Blocking of BMP signaling, but not of nodal signaling, inhibits autochthonous neural crest migration in zebrafish embryos. Blocking of BMP signaling,

Fig. 7. Lhx1-RNAi reduces the eye size

A model of signaling events regulating zebrafish fin regeneration.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 1. Aboveground biomass of Caragana and herbaceous plants, and proportional abundance of Caragana, under different grazing management treatments. Aboveground.

Integrin binding of soluble GRGDSP and fibronectin.

Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3.

Elevated expression of vegfaa in hemangiosarcoma.

Bivariate plots of mean total scratches and mean total pits for natural grazers. Bivariate plots of mean total scratches and mean total pits for natural.

Simultaneous perturbations to shuttling result in defects in the Dl gradient and in the sna domain. Simultaneous perturbations to shuttling result in defects.

Expression of Wnt3EGFP in the cerebellum of Tg(wnt3:Wnt3EGFP)F3 partially compensates cerebellum growth in MO1-injected Wnt3 morphants. Expression of Wnt3EGFP.

BMP signalling is dispensable for early gastruloid patterning.

Fig. 4. SMXL6 is degraded in response to SL treatment.

Statistical chart of significantly differentially expressed genes

Axial organisation of gastruloids.

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

Nodal signalling is absolutely required for T/Bra induction and correct patterning. Nodal signalling is absolutely required for T/Bra induction and correct.

Chronic phenytoin increases activity levels in Mecp2Null/Y mice.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Effects of fAS stimulation on cathepsin-B activity, mitochondrial quality and microglial cell survival after autophagy inhibition. Effects of fAS stimulation.

EpiDEG efficiently degrades GFP-tagged proteins that localize to different subcellular localizations. epiDEG efficiently degrades GFP-tagged proteins that.

Fig. 2. RFC3 function in chloroplasts.

Fig. 5. Flatworm density and activity on coral polyps

Two domains of KHC act to position the nucleus.

Wnt/β-catenin signalling stabilises and enhances spontaneous symmetry-breaking and polarisation events in gastruloids. Wnt/β-catenin signalling stabilises.

Effect of chronic disturbance and acute stressor on testosterone levels. Effect of chronic disturbance and acute stressor on testosterone levels. (A) Testosterone.

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.

Delay of tail resorption in trβ crispants during natural metamorphosis

Relative expression of clock genes in cultured goldfish liver treated with ghrelin, adenylate cyclase (AC) activator and protein kinase A (PKA) inhibitor.

Relative expression of clock genes in cultured goldfish liver treated with ghrelin and phospholipase C (PLC) inhibitor. Relative expression of clock genes.

Fig. 2. Coupling of actin to cell–cell junctions requires α-catenin and is necessary for the establishment of the barrier.(A) Effect of Cytochalasin D.

Morphological changes induced by T3 treatment in trβ crispants

Peroxisome speeds were slower in patient and control cells.

Fig. 2. Acetylation stiffens primary cilia.

Fig. 8. Compared mobilities of passenger proteins in G2/M-prophase, metaphase and anaphase.FRAP experiments were performed on HeLa cells stably expressing.

Percent change in behavioral dominance index and levels of plasma cortisol in descenders, non-descenders and controls. Percent change in behavioral dominance.

Biphasic increase of PI(4,5)P2 level in LPS-stimulated cells.

Depletion of COPI protein inhibits cis to trans cisternal maturation.

Degradation of the COPI proteins Ret1 and Sec21 by the AID system.

Fig. 1. Microarray analyses of genes whose expression is regulated by innervation during synaptogenesis.(A) Schematic drawings of the experimental design.

Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.

Fig. 8. Expression of other genomic-clustered Chrn subunits in the mesodiencephalon.(A) Schematic representation illustrating the assembly of the Chrnb4,

BCL-3 regulates expression of stemness-associated Wnt targets.

A statistic for microtubule order in mitotic spindles.

AP2μ2 has an essential function in Wnt/β-Catenin signaling.

Presentation transcript:

Wnt/β-catenin inhibition delays but does not inhibit T/Bra::GFP expression. Wnt/β-catenin inhibition delays but does not inhibit T/Bra::GFP expression. (A,B) T/Bra::GFP gastruloids stimulated with a pulse of Chi (48-72 h AA) following pre-treatment with vehicle IWP2, XAV939, DKK or Wnt3a (n=14 per condition). Fluorescence traces (A) and heatmaps of the data (B) are shown. Blocking secretion of Wnt proteins with IWP2 effectively abolishes T/Bra::GFP expression until 96 h AA, whereby highly heterogeneous expression is observed. Interestingly, the pulse of Chi can partially rescue T/Bra::GFP expression at 72 h following XAV939 pre-treatment, indicating the requirement for Wnt protein secretion in maintenance of expression. Wnt3a pre-treatment reduces the heterogeneity of the response, better defines the pole of expression and maintains high T/Bra expression for longer than controls (see Figs S7 and S8 for further details and statistical analysis). David A. Turner et al. Development 2017;144:3894-3906 © 2017. Published by The Company of Biologists Ltd