Glucose metabolic index measured using 2-deoxyglucose (2[3H]DG) was measured for the gastrocnemius and superficial vastus lateralis (SVL) during the last 30 min of a 120-min saline infusion or hyperinsulinemic-euglycemic clamp (4.0 mU kg–1 min–1) experiment on conscious, unrestrained mice fasted for 5 h. Glucose metabolic index measured using 2-deoxyglucose (2[3H]DG) was measured for the gastrocnemius and superficial vastus lateralis (SVL) during the last 30 min of a 120-min saline infusion or hyperinsulinemic-euglycemic clamp (4.0 mU kg–1 min–1) experiment on conscious, unrestrained mice fasted for 5 h. Wild-type (WT) or HK II overexpressing (HKTg) mice were fed either a standard diet or a high-fat diet up to the age of 4 months of age and fasted for 5 h. Data are means ± s.e.m. for 7–14 mice per group. *P<0.05 vs saline condition; †P<0.05 vs WT standard diet; ‡P<0.05 vs HKTg standard diet. Data are from Fueger et al. (Fueger et al., 2004b). Figure reproduced from Wasserman (Wasserman, 2009). David H. Wasserman et al. J Exp Biol 2011;214:254-262 © 2011.