Viability and function of the cryopreserved whole rat ovary: comparison between slow- freezing and vitrification Milan Milenkovic, M.D., Ph.D., César.

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Presentation transcript:

Viability and function of the cryopreserved whole rat ovary: comparison between slow- freezing and vitrification Milan Milenkovic, M.D., Ph.D., César Diaz-Garcia, M.D., Ann Wallin, B.Sc., Mats Brännström, M.D., Ph.D. Fertility and Sterility Volume 97, Issue 5, Pages 1176-1182 (May 2012) DOI: 10.1016/j.fertnstert.2012.01.123 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Schematic drawing of the abdominal vascular anatomy of the rat with the right ovary to be isolated. The rectangles represent points where vessels were ligated. The closed circle on the aorta indicates the insertion point of the cannula, and the open circle indicates the point where the cava was opened by a small slit. Fertility and Sterility 2012 97, 1176-1182DOI: (10.1016/j.fertnstert.2012.01.123) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 The number (median and ranges) of primordial and primary follicles within a cortex area of 0.06 mm2 in ovaries (n = 5 in each group) that were fresh (F) or cryopreserved either by vitrification in 1.5 M DMSO (L-VIT) or 7 M DMSO (H-VIT), or cryopreserved by slow freezing in 1.5 M DMSO (L-SLF) or 7 M DMSO (H-SLF). Statistical differences are indicated by brackets. Fertility and Sterility 2012 97, 1176-1182DOI: (10.1016/j.fertnstert.2012.01.123) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Levels of estradiol (median and ranges) in medium after incubation of ovarian tissue with 5 ng/mL human recombinant follicle-stimulating hormone (FSH) for 24 hours (n = 5 per group). The ovarian tissues were either fresh (F) or cryopreserved by vitrification in 1.5 M DMSO (L-VIT) or 7 M DMSO (H-VIT), or by slow freezing in 1.5 M DMSO (L-SLF) or 7 M DMSO (H-SLF). Statistical differences are indicated by brackets. Fertility and Sterility 2012 97, 1176-1182DOI: (10.1016/j.fertnstert.2012.01.123) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Histologic section of areas with small follicles of ovaries that had been cryopreserved in 1.5 M dimethyl sulfoxide (DMSO) by either (A) vitrification (L-VIT) or (B) slow freezing (L-SLF). Immunohistochemical staining against caspase is indicated by red stain (original magnification: ×200). Fertility and Sterility 2012 97, 1176-1182DOI: (10.1016/j.fertnstert.2012.01.123) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions