Volume 76, Issue 8, Pages (October 2009)

Slides:



Advertisements
Similar presentations
Julio P. Juncos, Joseph P. Grande, Narayana Murali, Anthony J
Advertisements

Inflammatory cytokines in acute renal failure
Volume 77, Issue 6, Pages (March 2010)
Volume 78, Issue 3, Pages (August 2010)
Volume 82, Issue 6, Pages (September 2012)
Inhibition of ILK by QLT-0267 suppresses type I and type III collagen expression and ameliorates interstitial collagen deposition and renal fibrosis after.
Volume 78, Issue 4, Pages (August 2010)
Volume 91, Issue 2, Pages (February 2017)
G.-T. Shin, D.R. Kim, J.-E. Lim, H. Yim, H. Kim  Kidney International 
Volume 86, Issue 1, Pages (July 2014)
Volume 81, Issue 9, Pages (May 2012)
Bax and Bak have critical roles in ischemic acute kidney injury in global and proximal tubule–specific knockout mouse models  Qingqing Wei, Guie Dong,
Volume 82, Issue 8, Pages (October 2012)
Volume 67, Issue 3, Pages (March 2005)
Volume 84, Issue 2, Pages (August 2013)
Volume 79, Issue 6, Pages (March 2011)
IN-1130, a novel transforming growth factor-β type I receptor kinase (ALK5) inhibitor, suppresses renal fibrosis in obstructive nephropathy  J.-A. Moon,
Volume 86, Issue 3, Pages (September 2014)
Volume 81, Issue 9, Pages (May 2012)
Volume 88, Issue 3, Pages (September 2015)
Volume 58, Issue 6, Pages (December 2000)
Mast cells decrease renal fibrosis in unilateral ureteral obstruction
Volume 75, Issue 2, Pages (January 2009)
Volume 67, Issue 1, Pages (January 2005)
Fenghua Zeng, Tomoki Miyazawa, Lance A. Kloepfer, Raymond C. Harris 
Volume 75, Issue 6, Pages (March 2009)
JAK/STAT signaling in renal diseases
Volume 73, Issue 4, Pages (February 2008)
Activation of hepatocyte growth factor receptor, c-met, in renal tubules is required for renoprotection after acute kidney injury  Dong Zhou, Roderick.
Volume 82, Issue 9, Pages (November 2012)
Volume 54, Issue 1, Pages (July 1998)
Volume 80, Issue 10, Pages (November 2011)
Heterozygous disruption of activin receptor–like kinase 1 is associated with increased renal fibrosis in a mouse model of obstructive nephropathy  José.
Autophagy in proximal tubules protects against acute kidney injury
Volume 81, Issue 12, Pages (June 2012)
Volume 84, Issue 2, Pages (August 2013)
Volume 84, Issue 5, Pages (November 2013)
Volume 63, Issue 2, Pages (February 2003)
Hepatocyte growth factor suppresses interstitial fibrosis in a mouse model of obstructive nephropathy  Shinya Mizuno, Kunio Matsumoto, Toshikazu Nakamura 
Volume 60, Issue 2, Pages (August 2001)
Volume 75, Issue 8, Pages (April 2009)
Volume 57, Issue 3, Pages (March 2000)
Treatment of chronic tubulointerstitial disease: A new concept
Volume 70, Issue 10, Pages (November 2006)
Volume 83, Issue 3, Pages (March 2013)
Volume 86, Issue 4, Pages (October 2014)
Aquaporin gene delivery to kidney
Deletion of the epidermal growth factor receptor in renal proximal tubule epithelial cells delays recovery from acute kidney injury  Jianchun Chen, Jian-Kang.
Volume 92, Issue 3, Pages (September 2017)
Volume 55, Issue 3, Pages (March 1999)
Genetic deficiency of adiponectin protects against acute kidney injury
Volume 80, Issue 11, Pages (December 2011)
Volume 68, Issue 2, Pages (August 2005)
Volume 85, Issue 4, Pages (April 2014)
Volume 67, Issue 2, Pages (February 2005)
Volume 75, Issue 6, Pages (March 2009)
Robert L. Chevalier, Michael S. Forbes, Barbara A. Thornhill 
Volume 82, Issue 2, Pages (July 2012)
Volume 83, Issue 6, Pages (June 2013)
Volume 68, Issue 2, Pages (August 2005)
Volume 79, Issue 2, Pages (January 2011)
M.-J. Wu, M.-C. Wen, Y.-T. Chiu, Y.-Y. Chiou, K.-H. Shu, M.-J. Tang 
Volume 54, Issue 1, Pages (July 1998)
TGF-β type II receptor deficiency prevents renal injury via decrease in ERK activity in crescentic glomerulonephritis  C.Y. Song, B.C. Kim, H.K. Hong,
Volume 58, Issue 5, Pages (November 2000)
Volume 84, Issue 1, Pages (July 2013)
J. Ma, A. Weisberg, J.P. Griffin, D.E. Vaughan, A.B. Fogo, N.J. Brown 
Volume 68, Issue 2, Pages (August 2005)
Volume 70, Issue 9, Pages (November 2006)
Volume 74, Issue 8, Pages (October 2008)
Presentation transcript:

Volume 76, Issue 8, Pages 868-876 (October 2009) Deletion of the Met receptor in the collecting duct decreases renal repair following ureteral obstruction  Hong Ma, Maryanna Saenko, Anthony Opuko, Akashi Togawa, Keita Soda, Arnaud Marlier, Gilbert W. Moeckel, Lloyd G. Cantley, Shuta Ishibe  Kidney International  Volume 76, Issue 8, Pages 868-876 (October 2009) DOI: 10.1038/ki.2009.304 Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 1 Semi-quantitative assessment of hydronephrosis. (a) Representative Trichrome staining of Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mouse kidney sections (UUO and contralateral control kidneys). (b). Quantification of the severity of hydronephrosis from (a). N=6. UUO, unilateral ureteral obstruction. Kidney International 2009 76, 868-876DOI: (10.1038/ki.2009.304) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 2 Quantitative and histological changes in the kidney medulla of Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice. (a–f). Real-time PCR for mRNA levels of the indicated factors in Metfl/fl;HoxB7-Cre kidneys expressed relative to Met+/+;HoxB7-Cre kidneys that have undergone UUO. N=7 for each factor. *P<0.01 vs Met+/+;HoxB7-Cre kidneys for type 1 collagen, *P<0.05 for type 4 collagen, and *P<0.05 for plasminogen activator inhibitor 1. (g). Representative Trichrome staining of Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice undergoing UUO at time points 1, 3, and 7 days. (h) Quantification of percent fibrosis in Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice following UUO. N=6. KO, knockout; UUO, unilateral ureteral obstruction; WT, wild type. Kidney International 2009 76, 868-876DOI: (10.1038/ki.2009.304) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 3 Comparison of acute tubular injury and interstitial infiltrate in Metfl/fl;HoxB7-Cre kidney vs Met+/+;HoxB7-Cre kidneys following UUO. (a) Quantification of percent interstitial infiltrate in Metfl/fl;HoxB7-Cre kidney vs Met+/+;HoxB7-Cre kidney. N=7. (b) Quantification of acute tubular injury for Metfl/fl;HoxB7-Cre kidney vs Met+/+;HoxB7-Cre kidney. N=7. UUO, unilateral ureteral obstruction. Kidney International 2009 76, 868-876DOI: (10.1038/ki.2009.304) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 4 Comparison of collecting duct apoptosis in Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice. (a) Representative staining of collecting duct cells for TdT-mediated dUTP nick end labeling (fluorescein isothiocyanate) and aquaporin-2 (Red) following UUO at days 1 and 3 in Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice. (b) Quantification of the number of apoptotic cells/aquaporin-2(AQP2) positive cells. N=7, *P<0.001 versus Met+/+;HoxB7-Cre mice at day 1 following UUO. UUO, unilateral ureteral obstruction. Kidney International 2009 76, 868-876DOI: (10.1038/ki.2009.304) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 5 Histological changes following reversal of UUO in Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice. (a) Representative Trichrome staining of Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre kidneys (contralateral control kidneys, UUO and reversal of UUO). (b) Kidney weight expressed as percentage of body weight in sham-operated mice, UUO, R-UUO N=6 *P<0.005 (Bar 6 compared with Bar 3). R-UUO, reversal of unilateral ureteral obstruction; WT, wild type. Kidney International 2009 76, 868-876DOI: (10.1038/ki.2009.304) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 6 Comparison of proliferation of collecting duct cells in Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice following reversal of unilateral ureteral obstruction (R-UUO). (a) Representative staining of collecting duct cells with Ki-67 and aquaporin-2 in contralateral control kidneys of Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice. (b) Representative staining of collecting duct cells with Ki-67 and aquaporin-2 following reversal of UUO in Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice. (c) Quantification of percentage Ki-67-positive and aquaporin-positive cells in the collecting ducts of Metfl/fl;HoxB7-Cre and Met+/+;HoxB7-Cre mice. N=6, P<0.001. (d). Blood urea nitrogen is elevated in Metfl/fl;HoxB7-Cre mice following reversal of UUO. N=6, *P<0.02. Kidney International 2009 76, 868-876DOI: (10.1038/ki.2009.304) Copyright © 2009 International Society of Nephrology Terms and Conditions