Fig. 8. Proposed scheme for DIF signaling pathways in D. discoideum.

Slides:

Advertisements

Similar presentations

Dictyostelium discoideum Slime Molds: The Social Amoebae 10 um 2 mm 10 5 cells.

Advertisements

The Chemotactic Paradox Amie Cubbon Denise Gutermuth.

Mathematical Biology Summer Workshop DICTYOSTELIUM DISCOIDEUM Social Amoeba –act like either a unicellular or multicellular organism depending on circumstances.

Lecture 9: Cell Communication I. Multicellular organisms need to coordinate cellular functions in different tissues Cell-to-cell communication.

Chemotaxis of Eukaryotic Cells: 1.Dictyostelium discoideum

Immuno-gold localisation of COPI in N. gruberi cells.

Cellular Signaling Ch. 11.

Signal trasduction via cAMP

Figure 2 Molecular pathways involved in the regulation of T-cell differentiation and cytokine production Figure 2 | Molecular pathways involved in the.

mTORC1 signalling links cellular growth with autophagy

Intracellular signalling: Sphingosine-1-phosphate branches out

Cellular signalling: Stressing the importance of PIP3

Amino acid-dependent regulation of autophagy by mTORC1

Cyclooxygenase-2 in the kidney: good, BAD, or both?

Intracellular signalling: Sphingosine-1-phosphate branches out

Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings.

Proposed model of signalling pathways involved in RBC shrinkage and vesiculation Inhibition of the depicted kinases induces shrinkage and vesiculation.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

A Drosophila cell culture model to study VAP(P58S) aggregation.

Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.

Fig. 6. Comparison between the response against transformed tissues and capsule formation.At the cellular level the two responses share many similarities.

Discordance and similarity between humans with FA and FA mouse models.

Colocalization of the palmitoylation-deficient mutants of claudin-14 with the lysosomal membrane protein LAMP-2. Colocalization of the palmitoylation-deficient.

Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Ingenuity pathway analysis of the genes enriched in both 96-h-induced MMs and human ccRCC. Ingenuity pathway analysis of the genes enriched in both 96-h-induced.

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Doxorubicin viability in the presence and absence of Herceptin

Dose-responsiveness and specificity of retinoic acid signaling sentinels. Dose-responsiveness and specificity of retinoic acid signaling sentinels. (A)

Fig. 5. GFP fluorescence colocalization of Gcn5.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 2. Blocking of BMP signaling, but not of nodal signaling, inhibits autochthonous neural crest migration in zebrafish embryos. Blocking of BMP signaling,

Fig. 2. The role of sma in cell migration

Fig. 4. One-dimensional gel electrophoresis of DIMs

Hypothetical model of HvAP2 control of stem elongation.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 8. Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine. Involvement.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Fig. 6. STK35 KO mice show ovary defects.

Fig. 1. Nrf2 affects the mitochondrial membrane potential

Fig. 3. Immunostaining for neural differentiation makers of the stage 29 optic cup.Immunoreactive signals are shown in green or red. Immunostaining for.

Statistical chart of significantly differentially expressed genes

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

Model of the Ang-1 pathway in early lung development.

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Fig. 5. Flatworm density and activity on coral polyps

Fig. 4. Autotoxicity triggered by withdrawal of antitoxin inducer in Synechocystis sp. Autotoxicity triggered by withdrawal of antitoxin inducer in Synechocystis.

The AUX and PIN auxin carrier proteins are transported by both ESCRT-dependent and ESCRT-independent pathways. The AUX and PIN auxin carrier proteins are.

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

Functions of AP-1 subunits in various cellular processes and disease.

Model for TGF-β-induced myofibroblast differentiation involving MKL1 isoform-specific activities. Model for TGF-β-induced myofibroblast differentiation.

The expression of two forms of N-cadherin.

Fig. 12. Overview of the molecular program essential to build mdDA neurons.The genes identified in this study (in red) have been added to the programming.

Schematic model of ERp44 regulation.

Fig. 3. transparent is required cell-autonomously in iridophores

Fig. 2. Expression of Cx43 mutant T154A resulted in non-radial spreading and formation of protrusions in J558µm3 cells spreading in response to BCR signaling.(A)

Fig. 8. Compared mobilities of passenger proteins in G2/M-prophase, metaphase and anaphase.FRAP experiments were performed on HeLa cells stably expressing.

Fig. 3. Mean force and velocity during jumping

mip120 null egg chambers have a condensed nurse cell DNA phenotype

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

Fig. 4. Representative still images of behaviour and characteristics typical of inshore foraging strategy of Australasian gannets. Representative still.

Fig. 1. Microarray analyses of genes whose expression is regulated by innervation during synaptogenesis.(A) Schematic drawings of the experimental design.

Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.

Fig. 5. Behaviours of the wild-types Oregon-R at two temperatures.

Fig. 3. Changes in the total EPS/Chl a ratio and bend interval of trichomes before and after the removal of polysaccharide from the BG11-cultured N. flagelliforme.

Fig. 1. lgl interacts genetically with Argonaute 1 (AGO1) in the eye.

Schematic model of the CD95/CD95L pathway and NO interference with cellular stress-pathway of apoptosis. Schematic model of the CD95/CD95L pathway and.

Schematic representation of signaling pathways associated with cannabinoid receptor activation induced by its agonists. Schematic representation of signaling.

Presentation transcript:

Fig. 8. Proposed scheme for DIF signaling pathways in D. discoideum. Proposed scheme for DIF signaling pathways in D. discoideum. (A) Hypothetical receptors for DIF-1 and DIF-2 control cell differentiation and chemotaxis. We assume that DIF-1 has two receptors: DR1-D (DR-1 in Kuwayama et al., 2011) and DR1-C (DR-2 in Kuwayama et al., 2011); DIF-2 has one receptor, DR2-C (DR-3 in Kuwayama et al., 2011). We hypothesize that (1) DIF-1 induces stalk cell differentiation via DR1-D (DIF-1 receptor responsible for induction of cell differentiation) and an increase in cytosolic calcium and proton concentrations (Kubohara and Okamoto, 1994; Kubohara et al., 2007); (2) DIF-1 suppresses chemotactic cell movement in shallow cAMP gradients via DR1-C (DIF-1 receptor responsible for modulation of chemotaxis) and the GbpB-dependent pathway; and (3) DIF-2 promotes chemotactic cell movement in shallow cAMP gradients via DR2-C (DIF-2 receptor responsible for modulation of chemotaxis) and the Dictyostelium histidine kinase C (DhkC)-RegA-dependent pathway (Kuwayama and Kubohara, 2016, 2009; Kuwayama et al., 2011). DhkC might function as DR2-C (Kuwayama and Kubohara, 2016). DIF-2 would also induce stalk cell differentiation via DR1-D (dotted arrow) due to its structural similarity to DIF-1. (B) Newly proposed scheme for chemotaxis modulation by DIF-1. DIF-1 localizes to mitochondria and suppresses chemotaxis in shallow cAMP gradients. Because CCCP and DNP, similar to DIF-1, suppress chemotaxis in shallow cAMP gradients, DIF-1 might suppress chemotactic cell movement via mitochondria and the GbpB-dependent pathway; DR1-C might reside in mitochondria. (C) Cellular localization of DIF-1 during early cell differentiation. In vegetative cells, DIF-1 penetrates the cell membrane but is continuously pumped out of the cells; in differentiating (aggregating) cells, DIF-1 is retained in some cells because of inactivation or disappearance of the pump. It is localized to mitochondria and promotes stalk cell differentiation. Yuzuru Kubohara et al. Biology Open 2017;6:741-751 © 2017. Published by The Company of Biologists Ltd