Characteristics of E. coli strains used in this study.

Slides:



Advertisements
Similar presentations
Bacterial Transformation RET Summer Overall Picture Bio-Rad pGLO Transformation Insertion of GFP gene into HB101 E. coli.
Advertisements

Vaccines development of Vibrio vulnificus
The Arabinose Operon Gene Regulation. Why Gene Regulation? Developmental Changes Cell Specialization Adaptation to the environment Prevents creation of.
First Cloning Experiment
Bacterial Plasmids Loops of DNA found in some bacteria
In the pGLO lab, we will: Use recombinant DNA Genetically engineer E. coli bacteria by inserting a plasmid Plate and grow bacteria Determine if the proteins.
Genetic Transformation of Bacteria and Gene Regulation.
PGLO Bacterial Genetics. E. Coli structure.
Bacterial Transformation
Genetic Transformation of Bacteria and Gene Regulation
PGLO Lab Purpose: To transform E. coli bacteria by adding plasmids that allow the bacteria to glow green under UV light in the presence of arabinose sugar.
Induction of apoptosis by CDIP expression.
Import of NH‐Idp3p‐, PTS‐1 (3HAD)‐ and PTS2 (3‐ketoacyl‐CoA thiolase)‐containing proteins in wild‐type, Δpex5 and Δpex7 cells. Import of NH‐Idp3p‐, PTS‐1.
Inhibition of ILK by QLT-0267 abrogates PAI-1 and MMP-2 induction by TGF-β1 in tubular epithelial cells. Inhibition of ILK by QLT-0267 abrogates PAI-1.
Volume 15, Issue 5, Pages (May 2016)
Defining the sequence within the TBSV p33 protein needed for binding to the Rsp5p WW-domain protein in vitro. Defining the sequence within the TBSV p33.
by Seokhee Kim, Juliana C. Malinverni, Piotr Sliz, Thomas J
Pirh2 promotes p73 ubiquitination in vivo.
التعلم بالإكتشاف المراجع:
Induction of cell filamentation by Fic-1.
Reduced copurification of selected co-opted host proteins with the viral replicase from yeast coexpressing WW-domain protein. Reduced copurification of.
Gene expression and immunologic consequence of SPAN-Xb in myeloma and other hematologic malignancies by Zhiqing Wang, Yana Zhang, Haichao Liu, Emanuela.
Dihydromyricetin decreases the sphere formation through downregulation of Sox2 in human osteosarcoma. Dihydromyricetin decreases the sphere formation through.
Testing the iGM insertion module.
Volume 58, Issue 3, Pages (May 2015)
Volume 49, Issue 3, Pages (February 2013)
Pglo LAB REFLECTION 1. WHAT WAS THE PURPOSE OF THIS LAB? DID YOU ACCOMPLISH IT? HOW DO YOU KNOW? 2. WHAT CONTROLS DID WE USE AND WHY? 3. WHAT GENES WERE.
pARA-R Sequence RFP Expression Sequence Biotechnology Lab Program
Cellular localization of the “writers” and “erasers” in U2OS-G3BP1 cells. Cellular localization of the “writers” and “erasers” in U2OS-G3BP1 cells. (A)
Sensor optimization for thiosulfate and tetrathionate detection in the gut Sensor optimization for thiosulfate and tetrathionate detection in the gut A–F(A.
H-NS2-FLAG expression is upregulated in DMEM and M9 minimal medium and when cells enter the stationary growth phase. H-NS2-FLAG expression is upregulated.
Schematic representation of Mal transport and regulon.
STING activation induces inhibition of the mTORC1 pathway.
Expression of SYCE2 inhibits the interaction of HP1α with H3K9me3.
Comparison of capsular polysaccharide production by AB (wt) and AB (ΔbfmR). Comparison of capsular polysaccharide production by AB
Volume 58, Issue 3, Pages (May 2015)
Flora Ambre Honoré, Vincent Méjean, Olivier Genest  Cell Reports 
Sucrose gradient analysis of ribosomes after repressing the genes for 60S r-proteins uL4 and uL22 and 60S assembly factors Rpf2 and Rrs1. Sucrose gradient.
Characteristics of the Camphor-Off switch.
Fur homodimerization and functional assays
T7 transcripts increase relative to E. coli transcripts over time.
MAPK14 regulates ROS generation in GS cells.
PDGFRα activity reduces phosphorylation of critical members of the translational initiation complex. PDGFRα activity reduces phosphorylation of critical.
Titration of antisera against the amount of extract loaded on Western blots. Titration of antisera against the amount of extract loaded on Western blots.
Expression of SvkA during development and induction of cytofission.
RECQL4 is a RanGTP dependent MAP
Data from in vitro competition experiments with E
Effect of 14 hit compounds on SMN protein expression in type I SMA patient fibroblast cells (GM03813). Effect of 14 hit compounds on SMN protein expression.
Effects of Z-FA-FMK on SMN protein expression in type II SMA patient fibroblast cells (GM22592). Effects of Z-FA-FMK on SMN protein expression in type.
Induction of MalE expression by Cpd-1 in RAM2808.
(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.
Stability of SsbA (A) and GFP (B) proteins in UA159 and ΔclpX strains.
Generation and genotyping of Syk-AQL mice.
Volume 121, Issue 2, Pages (April 2005)
Distribution of specific proteins of various cell fractions and standard calibration curves of Cpd-1. Distribution of specific proteins of various cell.
Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.
Interaction of NM-GFPagg with G3BP and p62.
Expression of selected genes in the ΔSMcomS strain in response to increasing concentrations of XIP. RT-PCR analysis of gene expression was performed in.
Mitotic control mutants have altered sensitivity to increased expression of flp1+. Mitotic control mutants have altered sensitivity to increased expression.
PROTEIN-PROTEIN INTERACTIONS
Expression of selected intergenic regions in response to increasing concentrations of XIP. qRT-PCR analysis of gene expression was performed with strain.
AnvM-dependent immune pathway in host cells.
TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.
The genes pykA and xylR are posttranscriptionally regulated by DicF.
E. coli RtcB efficiently mediate HAC1/XBP1 splicing in yeast
SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.
Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.
Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter. Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter.
Expression and functional characterization of Cu transporters CtrA2 and CtrC in A. nidulans. Expression and functional characterization of Cu transporters.
Mixed head-to-head competitions.
Presentation transcript:

Characteristics of E. coli strains used in this study. Characteristics of E. coli strains used in this study. (A) Characteristics of E. coli strains used in this study. The strains and plasmids were built and generously donated by the Misra's lab (33, 39). (B) Detection of the presence of the major porins, OmpC, and OmpF, and the maltoporin LamB in various strains grown in LB with or without 0.2% arabinose (Ara, induction of the LamB expression) by Western blots. Estelle Dumont et al. LSA 2019;2:e201800242 © 2018 Dumont et al.