Identification of X chromosome copies by quantitative real-time polymerase chain reaction for population screening tests Ester S. Ramos, M.D., Ph.D.,

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Identification of X chromosome copies by quantitative real-time polymerase chain reaction for population screening tests Ester S. Ramos, M.D., Ph.D., Juliana V. Serafim, B.Sc., Paula L. Takeuchi, Ph.D., Cintia R. Marcondes, Ph.D., Adriane Araújo, M.Sc. Fertility and Sterility Volume 94, Issue 6, Pages 2476-2478 (November 2010) DOI: 10.1016/j.fertnstert.2010.04.008 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Amplification plot showing the difference of one cycle between the female and male AR-exon 5 amplification (note that the threshold cycle [Ct] of both sexes for the GAPDH gene is the same). (B) Reference range (2 SD) for the AR copy number of the normal men (group 46,XY) and women (group 46,XX), using the DNA sample of a normal man (46,XY) as control (= 1). (C and D) Relative amplification of the control sample (46,XY control, RQ = 1), normal men (46,XY a and b) and women (46,XX), and patients with 45,X[100], 47,XXY[100], and 47,XXX[24]/46,XX[76] karyotypes. RQ = 2-▵▵Ct, AR = androgen receptor gene or target gene. See text for additional explanations. Fertility and Sterility 2010 94, 2476-2478DOI: (10.1016/j.fertnstert.2010.04.008) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions