Fig. 2. Kinetics of OptoEphB2 activation.

Slides:

Advertisements

Similar presentations

Rap1 regulates the accumulation of AJ material during ZA morphogenesis

Advertisements

Volume 68, Issue 3, Pages (November 2010)

Volume 22, Issue 16, Pages (August 2012)

Single-Photon Absorptions Evoke Synaptic Depression in the Retina to Extend the Operational Range of Rod Vision Felice A. Dunn, Fred Rieke Neuron Volume.

Volume 15, Issue 24, Pages (December 2005)

Volume 111, Issue 2, Pages (July 2016)

Heather Heerssen, Richard D. Fetter, Graeme W. Davis Current Biology

Sung E. Kwon, Edwin R. Chapman Neuron

Strong G-Protein-Mediated Inhibition of Sodium Channels

KIN-3 depletion leads to increased levels of centrosomal ZYG-1

CK2 is required for early cell divisions in C. elegans embryos

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Fig. 2. Outline of the two types of stimulus sequences employed in the analysis.(A) Environment information stimuli; (B) adaptation stimuli. Outline of.

Fig. 8. CCA and ChQ treatment induce accumulation of F-actin rings.

Fig. 4. E-cadherin expression level affects monomer dynamics.

Fig. 1. Chlamydia infection causes elevated levels of sortilin.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Dictyostelium expressing N-APC lack the ability to maintain direction during migration. Dictyostelium expressing N-APC lack the ability to maintain direction.

Expression of Shh-Gli target genes in astrocyte progenitors.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.

Fig. 1. Loss of PC following INT depletion

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Fig. 1. E-cadherin localizes in nano-scale clusters.

Fig. 5. Recovery steps of mitotic acentriolar cells after cold MT depolymerisation.(A,B) Time-lapse sequences of mitotic spindle re-formation at 18°C.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Fig. 3. Lifetime fecundity and fecundity across age blocks in flies from the selected and control populations emerging in the morning and evening windows.(a)

Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.

Fig. 2. Ex vivo inducible knockout of PDCD2 in ESCs results in loss of S phase entry and increased p53.(A) Growth curve of inducible knockout and WT ESCs.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

MiR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. miR-200c/141 overexpression in LS-8 cells induces E-cad expression.

Fig. 6. STK35 KO mice show ovary defects.

BMP signalling is dispensable for early gastruloid patterning.

Multiple-exon skipping.

Table 1. Average ± S.E. of level of dissimilarity scores of each feature per stripe per pattern comparison of sides of the same fish (“Same Individual”),

The idefix phenotype first becomes visible during metamorphosis

Volume 23, Issue 7, Pages (July 2016)

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Fig. 1. Loss of PC following INT depletion

Fig. 3. Enhanced EGFR signaling in Rhbdf2P159L/P159L mice.

Fig. 1. Optogenetic activation of EphB2.

Fig. 5. Flatworm density and activity on coral polyps

Analysis of Cdc7p at the end of meiosis.

Wnt/β-catenin signalling stabilises and enhances spontaneous symmetry-breaking and polarisation events in gastruloids. Wnt/β-catenin signalling stabilises.

Differential optogenetic stimulation of Rac and Cdc42 induces lamellipodia and directed migration on a FN substrate. Differential optogenetic stimulation.

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

ITSN1 recruitment stimulates cellular FN deposition to stabilize lamellipodia. ITSN1 recruitment stimulates cellular FN deposition to stabilize lamellipodia.

Stimulation of cells with FFAR4 agonist.

Fig. 2. Coupling of actin to cell–cell junctions requires α-catenin and is necessary for the establishment of the barrier.(A) Effect of Cytochalasin D.

Fig. 3. transparent is required cell-autonomously in iridophores

Fig. 2. Expression of Cx43 mutant T154A resulted in non-radial spreading and formation of protrusions in J558µm3 cells spreading in response to BCR signaling.(A)

Altered intracellular distributions of acetylated α-tubulin, mitochondria and peroxisomes. Altered intracellular distributions of acetylated α-tubulin,

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Fig. 8. Compared mobilities of passenger proteins in G2/M-prophase, metaphase and anaphase.FRAP experiments were performed on HeLa cells stably expressing.

Integrin β3 is dispensable for RhoA activity and contractility.

The vps13 mutants are hypersensitive to SDS but cell wall integrity is not compromised. The vps13 mutants are hypersensitive to SDS but cell wall integrity.

Running on the treadmill prolonged TrkB activation induced by exogenous BDNF in vivo. Running on the treadmill prolonged TrkB activation induced by exogenous.

Fig. 3. Mean force and velocity during jumping

Nutrient availability regulates growth of the X. laevis optic tectum.

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

Fig. 3. Inclusion of E-cadherin into stationary clusters requires cis-, trans-, and cytoplasmic interactions. Inclusion of E-cadherin into stationary clusters.

GOLPH3 interacts with COG complex proteins.

APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

Fig. 6 The C9ORF72/SMCR8 complex regulates ULK1.

Volume 15, Issue 24, Pages (December 2005)

Presentation transcript:

Fig. 2. Kinetics of OptoEphB2 activation. Kinetics of OptoEphB2 activation. (A) Left: western blot (pTyr) of MEF cell lysates after specified time of blue light illumination. Right: quantification of normalized total pTyr immunoactivity in cell lysates (n=3). Solid line denotes exponential fit with a time constant of 49.7 s. (B) OptoEphB2 cluster density (# of cluster/cell area, n=4) in MEFs under blue light activation. The dotted line denotes exponential fit with a time constant of ∼15 s. (C) Quantification of MEF cell rounding kinetics. Cell area was normalized to the mean value prior to photoactivation. Both cells expressing OptoEphB2 (black line, n=6) and the control cells expressing Kinase-dead mutant (gray line, n=7) were show for comparison. (D) Testing of the reversibility of OptoEphB2-induced cell rounding. MEFs expressing OptoEphB2 were stimulated with multiple trains of blue light (6×100-ms pulses, 0.1 Hz), while cell morphology was monitored with TIRFM. Top panel shows selected frames in time-lapse data, showing MEF contraction and recovery after the first round of stimulations, as well as the re-contraction after the second round of stimulations. Bottom panel shows the quantification cell area over time, normalized to the average cell area prior to the first stimulation (2 min). Blue bars denote time for optical stimulation. All error bars denote s.e.m. Clifford Locke et al. Biology Open 2017;6:1820-1830 © 2017. Published by The Company of Biologists Ltd