Nodal signalling is absolutely required for T/Bra induction and correct patterning. Nodal signalling is absolutely required for T/Bra induction and correct.

Slides:

Advertisements

Similar presentations

Sec1p requires Boi1/2p for proper localization.

Advertisements

Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn. Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn.

Aggregation is not required for cytoplasmic relocalization induced by misfolding mutations. Aggregation is not required for cytoplasmic relocalization.

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. Retinoic acid sentinels in live embryos and responding.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 2. Proportion of motile objects and track length

OA and related amines modulate motility and length of schistosomula.

Fig. 6. Comparison between the response against transformed tissues and capsule formation.At the cellular level the two responses share many similarities.

Fig. 4. Induction of a systemic immune response in RasV12-expressing larvae.(A) List of genes that are significantly induced (at q

Table 1. Menthol preference index (MPI) and average number of eggs laid per female (EPF) in F0 and F1 lines.The statistical significance of MPI was assessed.

Fig. 4. BMP and nodal induce invasion of metastatic and radial growth phase melanoma cells in human epidermal skin reconstructs. BMP and nodal induce invasion.

Positive relationship between the residual breaking force and cuticle area of lamellar joints. Positive relationship between the residual breaking force.

Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.

Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.

Identification of aging-related genes and affected biological processes. Identification of aging-related genes and affected biological processes. (A) Experimental.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 2. Immunopurified MRE11 removes topoisomerase IIα covalent complexes from genomic DNA.(A) Western blot probing K562 whole cell extract, K562 MRE11.

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. Pigmentation and melanophore counts of rainbow trout parr and smolt caudal fins.Pigmentation of (A) parr and (B) smolt. Pigmentation and melanophore.

eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Dose-responsiveness and specificity of retinoic acid signaling sentinels. Dose-responsiveness and specificity of retinoic acid signaling sentinels. (A)

Fig. 5. GFP fluorescence colocalization of Gcn5.

Fig. 2. Blocking of BMP signaling, but not of nodal signaling, inhibits autochthonous neural crest migration in zebrafish embryos. Blocking of BMP signaling,

Fig. 7. Lhx1-RNAi reduces the eye size

Superimposed traces representing the effects of different concentrations of adrenaline on AP characteristics. Superimposed traces representing the effects.

Hypothetical model of HvAP2 control of stem elongation.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Fig. 4. Co-immunostaining of nocodazole or ASNase treated RPE-1 cells with anti-hASNS and anti-alpha tubulin showed defect in both mitotic spindle formation.

Bivariate plots of mean total scratches and mean total pits for natural grazers. Bivariate plots of mean total scratches and mean total pits for natural.

Simultaneous perturbations to shuttling result in defects in the Dl gradient and in the sna domain. Simultaneous perturbations to shuttling result in defects.

BMP signalling is dispensable for early gastruloid patterning.

Fig. 7. A putative model for primarily describing the influence of external factors on the morphogenetic process of natural colonies of N. flagelliforme.

Distribution and extent of expression.

Statistical chart of significantly differentially expressed genes

Wnt/β-catenin inhibition delays but does not inhibit T/Bra::GFP expression. Wnt/β-catenin inhibition delays but does not inhibit T/Bra::GFP expression.

Axial organisation of gastruloids.

Fig. 2. Kinetics of OptoEphB2 activation.

Src tethers Cas to adhesion complexes.

Fig. 11. Chrna6 and Chrnb3 expression depends Pitx3 and Nurr1 but through a different mechanism reported for Vmat2.(A) QPCR analysis of Chrna6 and Chrnb3.

Mechanism of Akt1 in promoting reprogramming.

ZPR3 interferes with the DRN/DRNL-REV interaction and inhibits STM expression. ZPR3 interferes with the DRN/DRNL-REV interaction and inhibits STM expression.

Wnt/β-catenin signalling stabilises and enhances spontaneous symmetry-breaking and polarisation events in gastruloids. Wnt/β-catenin signalling stabilises.

Effect of chronic disturbance and acute stressor on testosterone levels. Effect of chronic disturbance and acute stressor on testosterone levels. (A) Testosterone.

Subdiffraction-limit imaging of BRCA1 and 53BP1 in IRIF

Representative force traces of the forelimb and hindlimb during steady-state locomotion. Representative force traces of the forelimb and hindlimb during.

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

AT1R–EGFR transactivation occurs in HMEC-LST-AT1R cells.

Fig. 3. Parp mutation rescues mitochondrial function in pink1 mutants.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Relative expression of clock genes in cultured goldfish liver treated with ghrelin and phospholipase C (PLC) inhibitor. Relative expression of clock genes.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Fig. 4. Visual and RPE phagocytic function in Best1Y227N mice.

Effects of Chd1-knockdown on global gene expression.

Morphological changes induced by T3 treatment in trβ crispants

Peroxisome speeds were slower in patient and control cells.

The influence of SHIP2 on cell migration in breast cancer cells.

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

Fig. 1. Microarray analyses of genes whose expression is regulated by innervation during synaptogenesis.(A) Schematic drawings of the experimental design.

Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.

Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.

Eps8 interacts with IRTKS and is involved in the generation of dorsal morphology. Eps8 interacts with IRTKS and is involved in the generation of dorsal.

FAK is required for LPA-mediated induction of trailing-edge retraction and the restoration of normal morphology. FAK is required for LPA-mediated induction.

Proliferative and morphological defects following TbCALM and TbEpsinR depletion. Proliferative and morphological defects following TbCALM and TbEpsinR.

Distinct mitochondrial membrane potential, ATP-to-ADP ratio and NAD(P)H responses to glucose and methylsuccinate. Distinct mitochondrial membrane potential,

A statistic for microtubule order in mitotic spindles.

Effect of mianserin (MI) on the stimulation of dancing by cocaine (COC). Effect of mianserin (MI) on the stimulation of dancing by cocaine (COC). Dance.

Presentation transcript:

Nodal signalling is absolutely required for T/Bra induction and correct patterning. Nodal signalling is absolutely required for T/Bra induction and correct patterning. (A) Gastruloids stimulated with Chi, SB43, Chi+SB43 or Nodal alone between 48 and 72 h AA (n=13, 14, 14, 14, respectively), or subjected to either vehicle or SB43 pre-treatment (24-48 h AA) prior to a Chi, Nodal or Chi+Nodal pulse (48-72 h AA; n=14 per condition). (B) Normalised fluorescence traces shown per condition with corresponding shape descriptors as heatmaps. SB43 treatment blocks the expression of T/Bra::GFP and cannot be rescued by Chi co-stimulation. Inhibition of Nodal signalling has a positive influence on axial length and elongation morphology, suggesting that Nodal modulates axial extension (see Figs S5 and S6 for further details and statistical analysis). David A. Turner et al. Development 2017;144:3894-3906 © 2017. Published by The Company of Biologists Ltd