Meta-analyses of two microbiome studies with heterogeneous variable 16S regions. Meta-analyses of two microbiome studies with heterogeneous variable 16S.

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Meta-analyses of two microbiome studies with heterogeneous variable 16S regions. Meta-analyses of two microbiome studies with heterogeneous variable 16S regions. (A) De novo tree construction resulted in strong artifacts in the PCoA space (see black arrow). (B) Insertion of heterogeneous sOTUs into the same backbone tree via SEPP resolved the artifact and enabled meaningful insights. (C) Available V2 reads from the “Yanomami” samples served as a positive control. Separation of samples from the two studies was indeed driven by body product and not by different sequencing parameters. Stefan Janssen et al. mSystems 2018; doi:10.1128/mSystems.00021-18