Interleukin 6 Indirectly Induces Keratinocyte Migration

Slides:



Advertisements
Similar presentations
IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
Advertisements

Antimicrobial Peptides Human β-Defensins Stimulate Epidermal Keratinocyte Migration, Proliferation and Production of Proinflammatory Cytokines and Chemokines 
Cdc42 Inhibits ERK-Mediated Collagenase-1 (MMP-1) Expression in Collagen-Activated Human Keratinocytes  Maryam G. Rohani, Brian K. Pilcher, Peter Chen,
Regulation of MAPK Activation, AP-1 Transcription Factor Expression and Keratinocyte Differentiation in Wounded Fetal Skin  Samantha Gangnuss, Allison.
Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor  Hiroshi Ishihara, Hiroshi Yoshimoto,
Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes  Genji Imokawa, Yutaka Takagi,
Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo  Eui Dong.
Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols Is Mediated Through DNA Repair and Subsequent Inhibition of Inflammation 
Regulation of Human Melanoma Growth and Metastasis by AGE–AGE Receptor Interactions  Riichiro Abe, Tadamichi Shimizu, Hiroshi Sugawara, Hirokazu Watanabe,
Accelerated Wound Repair in ADAM-9 Knockout Animals
Glu-Leu-Arg-Negative CXC Chemokine Interferon γ Inducible Protein-9 As a Mediator of Epidermal–Dermal Communication During Wound Repair  Latha Satish,
Keratinocyte Growth Regulation in Defined Organotypic Cultures Through IL-1-Induced Keratinocyte Growth Factor Expression in Resting Fibroblasts  Nicole.
IFNλ Stimulates MxA Production in Human Dermal Fibroblasts via a MAPK-Dependent STAT1-Independent Mechanism  Adewonuola A. Alase, Yasser M. El-Sherbiny,
Role of Monocyte Chemoattractant Protein-1 and its Receptor,CCR-2, in the Pathogenesis of Bleomycin-Induced Scleroderma  Toshiyuki Yamamoto, Kiyoshi Nishioka 
Combining the Multitargeted Tyrosine Kinase Inhibitor Vandetanib with the Antiestrogen Fulvestrant Enhances Its Antitumor Effect in Non-small Cell Lung.
Differences in Expression of Specific Biomarkers Distinguish Human Beard from Scalp Dermal Papilla Cells  Susan E. Rutberg, Meredith L. Kolpak, John A.
Elastin-Derived Peptides Upregulate Matrix Metalloproteinase-2-ediated Melanoma Cell Invasion Through Elastin-Binding Protein  Carole Ntayi, Anne-Laure.
Stefan W. Stoll, Jessica L. Johnson, Yong Li, Laure Rittié, James T
IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway  Hye Jung Kim, Tae-Yoon Kim  Journal.
Type II Collagen Accumulation in Overlying Dermo-Epidermal Junction of Pilomatricoma Is Mediated by Bone Morphogenetic Protein 2 and 4  Hideki Mieno,
Suppression of Vitamin D Receptor and Induction of Retinoid X Receptor α Expression During Squamous Differentiation of Cultured Keratinocytes  Siegfried.
Green Tea Polyphenols Prevent Ultraviolet Light-Induced Oxidative Damage and Matrix Metalloproteinases Expression in Mouse Skin  Praveen K. Vayalil, Anshu.
IL-31 Induces Chemotaxis, Calcium Mobilization, Release of Reactive Oxygen Species, and CCL26 in Eosinophils, Which Are Capable to Release IL-31  Nikola.
Characterization of Glucose Transport System in Keratinocytes: Insulin and IGF-1 Differentially Affect Specific Transporters  Shlomzion Shen, Sanford.
Gangliosides GD1b, GT1b, and GQ1b Suppress the Growth of Human Melanoma by Inhibiting Interleukin-8 Production: the Inhibition of Adenylate Cyclase1 
The Glutamate Release Inhibitor Riluzole Decreases Migration, Invasion, and Proliferation of Melanoma Cells  Maithao N. Le, Joseph L.-K. Chan, Stephen.
Role of p38 MAPK in UVB-Induced Inflammatory Responses in the Skin of SKH-1 Hairless Mice  Arianna L. Kim, Jeffrey M. Labasi, Yucui Zhu, Xiuwei Tang,
High Invasive Melanoma Cells Induce Matrix Metalloproteinase-1 Synthesis in Fibroblasts by Interleukin-1α and Basic Fibroblast Growth Factor-Mediated.
Topical Imiquimod Treatment Prevents UV-Light Induced Loss of Contact Hypersensitivity and Immune Tolerance  Thomas H. Thatcher, Irina Luzina, Rita Fishelevich,
In Vitro Keratinocyte Dissociation Assay for Evaluation of the Pathogenicity of Anti- Desmoglein 3 IgG Autoantibodies in Pemphigus Vulgaris  Ken Ishii,
All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes 
The Function of Nitric Oxide in Wound Repair: Inhibition of Inducible Nitric Oxide- Synthase Severely Impairs Wound Reepithelialization  Birgit Stallmeyer,
Rosiglitazone Inhibits Proliferation, Motility, and Matrix Metalloproteinase Production in Keratinocytes  Narasimharao Bhagavathula, Kamalakar C. Nerusu,
Halofuginone, an Inhibitor of Type-I Collagen Synthesis and Skin Sclerosis, Blocks Transforming-Growth-Factor-β-Mediated Smad3 Activation in Fibroblasts 
The Suppressor of Cytokine Signaling (SOCS)-3 Determines Keratinocyte Proliferative and Migratory Potential during Skin Repair  Andreas Linke, Itamar.
The Hyaluronan Synthesis Inhibitor 4-Methylumbelliferone Prevents Keratinocyte Activation and Epidermal Hyperproliferation Induced by Epidermal Growth.
Imre Szabo, Michele A. Wetzel, Thomas J. Rogers 
Signaling Events During Induction of Plasminogen Activator Inhibitor-1 Expression by Sphingosylphosphorylcholine in Cultured Human Dermal Fibroblasts 
The p38-MAPK/SAPK Pathway is Required for Human Keratinocyte Migration on Dermal Collagen  Wei Li, Celina Nadelman, Ginard Henry, Jianhua Fan, Matt Muellenhoff,
Transforming Growth Factor β1 Induces Apoptosis in Normal Melanocytes but not in Nevus Cells Grown in Type I Collagen Gel  Tuomo Alanko  Journal of Investigative.
Heat Shock-Induced Matrix Metalloproteinase (MMP)-1 and MMP-3 Are Mediated through ERK and JNK Activation and via an Autocrine Interleukin-6 Loop  Chi-Hyun.
Gottron's Papules Exhibit Dermal Accumulation of CD44 Variant 7 (CD44v7) and Its Binding Partner Osteopontin: A Unique Molecular Signature  Jessica S.
IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
Differential Roles of Insulin Receptor and Insulin-Like Growth Factor-1 Receptor in Differentiation of Murine Skin Keratinocytes  Efrat Wertheimer, Meirav.
Th2 Cytokines Suppress Lipoteichoic Acid–Induced Matrix Metalloproteinase Expression and Keratinocyte Migration in Response to Wounding  Anne M. Brauweiler,
Bcl-2 Reduced and Fas Activated by the Inhibition of Stem Cell Factor/KIT Signaling in Murine Melanocyte Precursors  Satoko Kimura, Tamihiro Kawakami,
Aloe Barbadensis Extracts Reduce the Production of Interleukin-10 After Exposure to Ultraviolet Radiation  Son Won Byeon  Journal of Investigative Dermatology 
A Novel Role of Fibrin in Epidermal Healing: Plasminogen-Mediated Migration and Selective Detachment of Differentiated Keratinocytes  David J. Geer, Stelios.
IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma  Takeshi Noma, MD, PhD, Izumi.
Jens Hasskarl, Palanivel Velupillai, Karl Münger 
In Vitro Differences Between Keratinocyte Stem Cells and Transit-Amplifying Cells of the Human Hair Follicle  Cecilia Roh, Qingfeng Tao, Christos Photopoulos,
James Gailit, Mary J. Marchese, Richard R. Kew, Barry L. Gruber 
Corticotropin-Releasing Hormone (CRH) Downregulates Interleukin-18 Expression in Human HaCaT Keratinocytes by Activation of p38 Mitogen-Activated Protein.
Mechanical Stretching In Vitro Regulates Signal Transduction Pathways and Cellular Proliferation in Human Epidermal Keratinocytes  Shoichiro Yano, Mayumi.
Keratinocytes Inhibit Expression of Connective Tissue Growth Factor in Fibroblasts In Vitro by an Interleukin-1α-Dependent Mechanism  Daniel Nowinski,
Ligation of the β4 Integrin Triggers Adhesion Behavior of Human Keratinocytes by an “Inside-out” Mechanism  Stefan Kippenberger, Stefan Loitsch, Jutta.
John M. Lamar, Vandana Iyer, C. Michael DiPersio 
Human Leukocyte Elastase Induces Keratinocyte Proliferation by Epidermal Growth Factor Receptor Activation  Ulf Meyer-Hoffert, Jana Wingertszahn, Oliver.
Ultraviolet B Radiation Upregulates the Production of Macrophage Migration Inhibitory Factor (MIF) in Human Epidermal Keratinocytes  Tadamichi Shimizu,
Relationship Between Cell-Associated Matrix Metalloproteinase 9 and Psoriatic Keratinocyte Growth  Nathalie Buisson-Legendre, Hervé Emonard, Philippe.
TSH Receptor and Thyroid-Specific Gene Expression in Human Skin
CD44 phosphorylation regulates melanoma cell and fibroblast migration on, but not attachment to, a hyaluronan substratum  David Peck, Clare M. Isacke 
Effects of Hepatocyte Growth Factor on the Expression of Type I Collagen and Matrix Metalloproteinase-1 in Normal and Scleroderma Dermal Fibroblasts 
The Interleukin-6 Cytokine System Regulates Epidermal Permeability Barrier Homeostasis  Xu-Ping Wang, Michael Schunck, Karl-Josef Kallen, Claudia Neumann,
Hyun Jeong Park, Hee Jung Kim, Jun Young Lee, Baik Kee Cho, Richard L
Regulation of IL-13 Receptors in Human Keratinocytes
Mechanism of Thymus- and Activation-Regulated Chemokine (TARC)/CCL17 Production and its Modulation by Roxithromycin  Mayumi Komine, Takashi Kakinuma,
Interleukin-17 is Produced by Both Th1 and Th2 Lymphocytes, and Modulates Interferon-γ- and Interleukin-4-Induced Activation of Human Keratinocytes  Cristina.
Fibroblast Growth Factor 10 Induces Proliferation and Differentiation of Human Primary Cultured Keratinocytes  Cinzia Marchese, Alessandra Felici, Vincenzo.
Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1 
Presentation transcript:

Interleukin 6 Indirectly Induces Keratinocyte Migration Randle M. Gallucci, Dusti K. Sloan, Julie M. Heck, Anne R. Murray, Sijy J. O'Dell  Journal of Investigative Dermatology  Volume 122, Issue 3, Pages 764-772 (March 2004) DOI: 10.1111/j.0022-202X.2004.22323.x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 IL-6 is not a mitogen for keratinocytes from IL-6 KO mice. Epidermal keratinocytes were isolated from IL-6 KO mice, and seeded on collagen I-coated 96-well plates. Cells were serum/growth factor starved for 4 h, treatments were applied in quadruplicate, and incubated 16–20 h at 37°C and 5% CO2. Following incubation, 10 μL of 0.1 mM BrdU was added to each well, and the plate incubated an additional 8 h. BrdU uptake was determined by ELISA according to the manufacturer's instructions (Roche, Indianapolis, Indiana). Results were obtained via an ELISA plate reader, and raw data expressed as mean±SEM (n=4) percent negative (non-treated) control. Journal of Investigative Dermatology 2004 122, 764-772DOI: (10.1111/j.0022-202X.2004.22323.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 IL-6 induces keratinocyte migration indirectly through a dermal cell-produced soluble factor. Dermal cells (primary fibroblasts) and epidermal keratinocytes from IL-6 KO mice were cultured in 24-well tissue culture dishes to approximately 80% confluency. Transwell culture inserts (8 μm, tissue culture treated) were placed in the wells, and IL-6 KO keratinocytes were seeded into the transwell enclosures. Cultures were cultured in complete media (KGM), or treated with the indicated concentrations of rmIL-6 for 16–18 h in serum-free/supplement-free media (SFKGM) at 37°C and 5% CO2. Following incubation, transwell membranes were stained with hematoxylin, and migrating cells were enumerated by light microscopy. Data are expressed as mean+/-SEM (n=7) number of cells counted in five random fields when viewed at ×20 magnification approximately 800μm (*significantly different from serum-free media control, p<0.05). Journal of Investigative Dermatology 2004 122, 764-772DOI: (10.1111/j.0022-202X.2004.22323.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Culture supernatants from rmIL-6 fibroblasts induce migration of keratinocytes. Dermal cells from IL-6 KO mice were grown in 75 cm2 tissue culture flasks until approximately 80% confluence, and then treated with the indicated concentrations of rmIL-6 for 16 h in SFKGM at 37°C and 5% CO2. IL-6-treated fibroblast culture supernatants (FCM) were collected and 0.2 μm filtered. FCM was placed in the wells of a 24-well plate, transwell culture inserts (8 μm, tissue culture treated) were placed in wells of the plate, and IL-6 KO keratinocytes were seeded into the transwell enclosures. Plates were allowed to incubate for 16–18 h, after which the transwell membranes were removed, fixed, stained with hematoxylin, and migrating cells were enumerated by light microscopy. Data are expressed as mean±SEM (n=4) number of cells counted in five random fields when viewed at ×20 magnification approximately 800μm (*significantly different from SFKGM control, p<0.05). Journal of Investigative Dermatology 2004 122, 764-772DOI: (10.1111/j.0022-202X.2004.22323.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Dermal cell culture supernatants induce migration of keratinocytes in an in vitro wound model. Keratinocytes from IL-6 KO mice, were grown to confluency on collagen I-coated tissue culture plates. An in vitro wound was administered to the culture (wound margin depicted as dotted line). The cultures were washed and incubated in SFKGM containing 10 ng per mL rmIL-6, or FCM for 24 h at 37°C and 5% CO2. Migrating cells were enumerated in three specific previously designated and photographed fields at ×20 magnification approximately 800μm. (A) Data are expressed as means±SE (n=4, *significantly different from SFKGM control, p<0.05). Keratinocyte cultures immediately after wounding: SFKGM (B) and FCM (C). Keratinocyte cultures 24 h following wounding: SFKGM (D) and FCM (E). Journal of Investigative Dermatology 2004 122, 764-772DOI: (10.1111/j.0022-202X.2004.22323.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Dermal culture supernatants do not induce proliferation of keratinocytes. Epidermal keratinocytes from IL-6 KO mice were seeded onto collagen I-coated 96-well culture plates and allowed to adhere for 4 h. The cells were washed twice with PBS and treatments were added in quadruplicate as indicated, and incubated for 16–18h. Following incubation, 10 μL of 0.1 mM BrdU was added to each well, and the cells were then incubated an additional 8 h. Medium was then aspirated from the wells and ELISA determined BrdU uptake according to the manufacturer's instructions (Roche). Results were obtained via an ELISA plate reader, and raw data expressed as mean±SEM (n=4) percent negative (non-treated) control (*significantly different from SFKGM control, p<0.05). Journal of Investigative Dermatology 2004 122, 764-772DOI: (10.1111/j.0022-202X.2004.22323.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 IL-6 is not a mitogen fibroblast from IL-6 KO mice. Dermal fibroblasts isolated from IL-6 KO mice were seeded at 1×104 per well on tissue culture-coated 96-well plates. The cells were serum/growth factor starved for 4 h, and treatments were applied in quadruplicate. Cells were incubated 16–20 h at 37°C and 5% CO2. BrdU or WST reagent (Roche) was added to the wells according to the manufacture's instructions. The plates were incubated an additional 3 h (WST) or 8 h (BrdU), and the assays were completed according to the manufacturer's instructions. Results were obtained via an ELISA plate reader, and raw data expressed as mean±SEM (n=4) percent negative (SFKGM) control. Light bars=BrdU, dark bars=WST1 reagent (*significantly different from SFKGM control, p<0.05). Journal of Investigative Dermatology 2004 122, 764-772DOI: (10.1111/j.0022-202X.2004.22323.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 IL-6 induces phosphorylation of STAT3 in fibroblasts from IL-6 KO mice. Primary dermal fibroblasts from IL-6 KO mice were grown to 80% confluence on 150 mm tissue culture plates, and serum/growth factor starved for 6 h. Treatments were applied as indicated. Nuclear protein was isolated from the treated cultures at the indicated time points. Western blot analysis was performed on 25 μg of nuclear protein, and bands were detected with anti-phosphoprotein antibodies (Cell Signaling, Beverly, Massachussets). Blots were imaged via an Odyssey fluorescent imager (LiCor, Lincoln, Nebreska). Photographs are representative of three identical experiments. Journal of Investigative Dermatology 2004 122, 764-772DOI: (10.1111/j.0022-202X.2004.22323.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions