Fig. 2. Examples of changes in angular velocity and correlation coefficient during the O–O test. Examples of changes in angular velocity and correlation.

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Fig. 2. Examples of changes in angular velocity and correlation coefficient during the O–O test. Examples of changes in angular velocity and correlation coefficient during the O–O test. The values in the first clockwise, second counterclockwise, and third clockwise stripe rotations are shown in red, green, and blue, respectively. The angular velocity became ±36°/s and the correlation coefficient became +1.0 when fish followed the rotating stripes exactly. (A,B) A typical example of OMR-positive fish (control fish at λ=750 nm). The fish quickly started and stably continued following the stripes in all three rotations. (C,D) A typical example of OMR-negative fish (mutant fish at λ=850 nm). The fish continued swimming slowly in a clockwise direction (without any turning) throughout the 90 s O–O test. (E,F) Examples of fish that did not follow the stripes. Mutant fish at λ=760 nm (E) immediately started the OMR in the first rotation, but not in the second or third rotations. Whether the fish overlooked or intentionally ignored the changes in rotating direction is unknown. Control fish at λ=720 nm (F) restlessly swam back and forth in the first and second rotations, whereas the correlation coefficient became stably high for at least ∼10 s in the third rotation. These quick changes in swimming direction were rarely observed when fish could not follow the stripes (see Fig. 2D). (G,H) Examples of fish with an ambiguous behavior. Whether medaka did not or could not follow the stripes was unclear. A control fish at λ=850 nm (G) largely swam in the rotating direction, but the values fluctuated comparison with those shown in Fig. 2B. A mutant fish at λ=750 nm (H) seemed to start following the stripes at the end of each rotation, but this could be an accidental correlation. Megumi Matsuo et al. Biology Open 2018;7:bio033175 © 2018. Published by The Company of Biologists Ltd