Electron tomography. Electron tomography. (A) Simian immunodeficiency virus viewed frozen hydrated and unstained in a cryo 300-kV transmission electron.

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Analysis of Purified SIV Virions by Cryo- Electron Tomography (A) Low-dose projection image of a plunge- frozen specimen. (B) A series of images recorded.

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Electron tomography. Electron tomography. (A) Simian immunodeficiency virus viewed frozen hydrated and unstained in a cryo 300-kV transmission electron microscope; glycoprotein spikes and the internal core are visible. (B) Four 1-nm-thick slices from a tomogram. (C) Computer-generated three-dimensional reconstruction of one viral particle seen in panel B. Bars, 50 nm. Magnification, ×100,000. (Reprinted from reference 85.)‏ Cynthia S. Goldsmith, and Sara E. Miller Clin. Microbiol. Rev. 2009; doi:10.1128/CMR.00027-09