Fig. 3. Overexpression of wild-type GFP-CPAP, but not GFP-CPAP-377EE (GFP-377EE), induces cilia formation and promotes the growth of cilia.CAD cells (A,B)

Slides:

Advertisements

Similar presentations

Sec1p requires Boi1/2p for proper localization.

Advertisements

Characterization of DPR proteins in primary rat neurons and HEK293 cells. Characterization of DPR proteins in primary rat neurons and HEK293 cells. GFP,

Volume 31, Issue 1, Pages (July 2001)

The PAR-6 Polarity Protein Regulates Dendritic Spine Morphogenesis through p190 RhoGAP and the Rho GTPase Huaye Zhang, Ian G. Macara Developmental Cell

Ectopic overexpression of WOR3 drives switching to the opaque cell type. Ectopic overexpression of WOR3 drives switching to the opaque cell type. (A) Visualization.

Inducible DM1 model displays increased autophagy.

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

Fig. 4. Clinically defined SMN mutants show alterations in the association with telomerase complex proteins. Clinically defined SMN mutants show alterations.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

RNAi screen for host factors involved in L. monocytogenes dissemination. RNAi screen for host factors involved in L. monocytogenes dissemination. (A) Images.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 4. Seeding density modulates cell shape in both cell types

Fig. 4. A primary screen based on scrape closure

A Drosophila cell culture model to study VAP(P58S) aggregation.

Fig. 2. Mapping of the interaction domain on coilin for association with the dyskerin complex. Mapping of the interaction domain on coilin for association.

Fig. 1. Exogenous folic acid and Folr1 rescues the function of a Rho-kinase binding mutation in Shroom3. Exogenous folic acid and Folr1 rescues thefunction.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.

Fig. 1. Loss of PC following INT depletion

IQGAPs localize at the tips of axons in hippocampal neurons.

Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.

Fig. 1. E-cadherin localizes in nano-scale clusters.

Fig. 4. Acentriolar mitotic spindle assembly

PfSec13 does not co-localize with P. falciparum heterochromatin.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

Fig. 1. Ovarian cancer spheroids can bud from a monolayer

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Fig. 4. Detection of dFMR1 mRNA in dFMRP granules by FISH

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 6. Localization of Sma during growth and migration

Fig. 5. GFP fluorescence colocalization of Gcn5.

Fig. 2. The localisation of integrins β1 and α6 in the hES cells grown in a colony and as a single-cell culture. hES cells were harvested manually as small.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 10. A mutant clone homozygous for mip120LL07629 has greatly diminished Mip120 protein levels. hsFLP/+; FRT42B, mip120LL07629/FRT42B, Ubi-GFP-nls females.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.

MiR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. miR-200c/141 overexpression in LS-8 cells induces E-cad expression.

MET activation increases the density of Syn-GFP/bassoon and Syn-GFP/PSD-95 clusters in neocortical neurons. MET activation increases the density of Syn-GFP/bassoon.

Fig. 2. Fluorescent images indicating the cytoskeleton of human bone marrow-derived mesenchymal stem cells (hBMSCs) subjected to cyclic stretching. Fluorescent.

The idefix phenotype first becomes visible during metamorphosis

Fig. 2. TSA impairs growth and disrupts morphogenesis of exocrine pancreas in zebrafish larvae with hyperacetylation of nucleosomal histones.WT zebrafish.

Fig. 8. The morphology of the ventral nerve cord in Ror-Myc overexpressing embryos is normal. The morphology of the ventral nerve cord in Ror-Myc overexpressing.

Fig. 4. Elevated levels of tRNAiMet promote migration and invasion of melanoma cells. Elevated levels of tRNAiMet promote migration and invasion of melanoma.

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Effect of the plastid translation inhibitor Spec on LR development

Fig. 4. Perinuclear dynein regulators are not required for primary ciliogenesis.RPE cells were transfected with siRNA and either nocodazole-treated, fixed,

Fig. 1. Loss of PC following INT depletion

Analysis of Cdc7p at the end of meiosis.

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

hADAR1 and hADAR2 co-localize within the nucleolus.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Wrch-1 localizes to focal adhesions.

Loss of Graf results in plasmatocyte overproliferation.

Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Fig. 1. p85β localizes at adhesion plaques and associates with FAK

Segments R1, R3, R6 and R7, and GTPase activity of Mfn2ΔTM are necessary for mitochondrial fragmentation in COS7 cells. Segments R1, R3, R6 and R7, and.

Fig. 5. EGL-20 inhibits anterior and posterior orientation of UNC-40 asymmetric localization and the formation of axons from these sites.(A–D) HSN neurons.

The BRCA1 aggregates exclude large nuclear structures.

mip120 null egg chambers have a condensed nurse cell DNA phenotype

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

The regulatory domain of HSF1 is involved in the pro-apoptotic response to TNF. (A) Upper panel, functional domains and potential DAPK phosphorylation.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Ubiquitin mediates the interaction between Smo and Vps36.

GFP-Myo10 overexpression increases TNT numbers in neuronal CAD cells.

Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM,

Fig.S1 A CD79a pMst1 overlay 0 min 5 min 10 min 30 min B C.

Presentation transcript:

Fig. 3. Overexpression of wild-type GFP-CPAP, but not GFP-CPAP-377EE (GFP-377EE), induces cilia formation and promotes the growth of cilia.CAD cells (A,B) and hippocampal neurons (C,D) were transfected with GFP-CPAP, GFP-377EE, or GFP vector alone (control) and then serum starved for 72 hours (CAD cells) or 4 DIV (hippocampal neurons). Overexpression of wild-type GFP-CPAP, but not GFP-CPAP-377EE (GFP-377EE), induces cilia formation and promotes the growth of cilia.CAD cells (A,B) and hippocampal neurons (C,D) were transfected with GFP-CPAP, GFP-377EE, or GFP vector alone (control) and then serum starved for 72 hours (CAD cells) or 4 DIV (hippocampal neurons). Images of CAD cells (A) and hippocampal neurons (C) labeled with DAPI and the indicated antibodies. GFP-CPAP and GFP-377EE were directly visualized by confocal fluorescence microscopy. Quantitative analysis of ciliated cell numbers and cilia length in CAD cells (B) and hippocampal neurons (D). Bar values are means ± s.d. of three experiments (n = 100 counted for ciliated cells; n = 20 counted for cilia length). *p < 0.05. Bar in A,C: 2 µm. Kuo-Sheng Wu, and Tang K. Tang Biology Open 2012;1:559-565 © 2012. Published by The Company of Biologists Ltd