Growth, adhesion, biofilm formation, and aggregation of WT R2866 and the R2866ΔampG gene deletion mutant. Growth, adhesion, biofilm formation, and aggregation.

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Growth, adhesion, biofilm formation, and aggregation of WT R2866 and the R2866ΔampG gene deletion mutant. Growth, adhesion, biofilm formation, and aggregation of WT R2866 and the R2866ΔampG gene deletion mutant. (A) Overnight growth determined by measuring OD620. (B) Biofilm formation determined by measuring crystal violet light A560. (C, D) Numbers of WT R2866 and R2866ΔampG gene deletion mutant CFU in medium (C) and biofilm (D). (E) Initial adhesion to a solid surface determined by the biofilm ring test after 2 and 4 h of static growth at 37°C. The BFI was adjusted by the test software and is inversely proportional to the number of adherent bacteria. (F) Percent adhesion of WT R2866 and R2866ΔampG gene deletion mutant bacteria to A549 lung epithelial cells after 2 to 3 h of incubation. (G) Aggregation of WT R2866 and the R2866ΔampG gene deletion mutant in 5 ml of sBHI medium as determined by OD620 after 0, 2, 4, and 8 h of static incubation. An unpaired Student t test (A to F) or two-way analysis of variance with the Bonferroni post hoc test (G) was used for statistical analysis (NS, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001). Sara Marti et al. mSphere 2017; doi:10.1128/mSphere.00329-16