Mechanisms of allergen-specific desensitization

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Presentation transcript:

Mechanisms of allergen-specific desensitization Christina Uermösi, MS, Roger R. Beerli, PhD, Monika Bauer, PhD, Vania Manolova, PhD, Klaus Dietmeier, PhD, Regula B. Buser, MS, Thomas M. Kündig, MD, Philippe Saudan, PhD, Martin F. Bachmann, PhD Journal of Allergy and Clinical Immunology Volume 126, Issue 2, Pages 375-383 (August 2010) DOI: 10.1016/j.jaci.2010.05.040 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 mAbs display similar binding properties to rFel d 1 and recognize distinct epitopes. A, Binding properties of the 3 mAb clones (A044 ♦, F127 ■, and G078 ▴) to monomeric rFel d 1, assessed by ELISA. The mAbs displayed similar properties of binding to monomeric rFel d 1, regardless of isotype. B, Sequential addition of the different clones and isotypes, as indicated in Methods, yielded results similar to those of any of the mAbs alone in a competition ELISA. These findings indicate that the clones recognize distinct epitopes on monomeric rFel d 1. Data represent 3 or more independent experiments. Journal of Allergy and Clinical Immunology 2010 126, 375-383DOI: (10.1016/j.jaci.2010.05.040) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Antibodies that recognize 2 epitopes suffice for BMMC degranulation. Balb/c BMMCs were loaded with 1, 2, or 3 of the indicated IgE clones. Degranulation was induced by monomeric (A) or dimeric (B) rFel d 1. C, Wild-type BMMCs were loaded with IgE A044 (●), IgE A044/F127 (), or IgE A044/F127/G078 (). Calcium flux was induced by addition of monomeric rFel d 1 and was measured by flow cytometry. D, Mice were given intravenous injections of 1, 2, or 3 of the indicated IgE antibodies. Mast cell degranulation was measured by intradermal ear prick test with monomeric rFel d 1. The results show the means ± SEMs of the OD595; data shown are representative for at least 3 independent experiments. Journal of Allergy and Clinical Immunology 2010 126, 375-383DOI: (10.1016/j.jaci.2010.05.040) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 A single IgG can inhibit mast cell degranulation. A, Balb/c BMMCs were loaded with IgE A044 and IgE F127 before the addition of monomeric rFel d 1, alone or in a complex with IgG1 G078 (left) or IgG2a G078 (right). B, IgGs bind mast cells only as preformed immune complexes with rFel d 1. Monomeric rFel d 1 was incubated with the indicated IgG clones and loaded on BMMCs. The binding of IgGs was analyzed by flow cytometry. Data shown are representative for 3 or more independent experiments. Journal of Allergy and Clinical Immunology 2010 126, 375-383DOI: (10.1016/j.jaci.2010.05.040) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 IgGs inhibit mast cell activation in a FcγRIIB-dependent fashion. A,FcγRIIB-/- BMMCs were loaded with IgE A044 and IgE F127. Degranulation was induced by incubation either with monomeric rFel d 1 alone or in a complex with IgG1 G078 (left) or IgG2a G078 (right). B, Balb/c BMMCs were loaded with IgE A044 and IgE F127. Degranulation was induced by incubation either with monomeric rFel d 1 alone or complexed with F(ab)2-G078. Balb/c (C) or FcγRIIB-/- (D) mice were given intravenous injections of IgE A044, IgE-F127, and IgG1 G078 or IgG2a G078. Mast cell degranulation was measured after an intradermal ear prick with monomeric rFel d 1. The results indicate the means ± SEMs, and data shown are representative for 3 independent experiments. Journal of Allergy and Clinical Immunology 2010 126, 375-383DOI: (10.1016/j.jaci.2010.05.040) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 A single IgG can inhibit basophil degranulation of cat allergic individuals. A-D, Heparinized whole blood from individuals with cat allergy was incubated with monomeric rFel d 1 (gray bars) or with indicated clones of IgG–rFel d 1 (black bars) IC (left, A-D) or IgG4-rFel d 1 IC (black bars; right panels, A-D). Enlargement of the IC by adding 2 (C) or 3 (D) different IgG1 or IgG4 clones (labelled below the respective graphs) increased the inhibition. The results show the means ± SEMs, and data are representative for 3 donors with cat allergies. E, Basophil degranulation was induced by incubation with IC of IgG1-G078 and monomeric (black bars) or dimeric (gray bars) rFel d 1. The values of the y-axis were normalized to 100% activation. One hundred percent activation was defined by basophils that had been challenged with monomeric rFel d 1. The results show the means ± SEMs; data shown are derived from 2 independent experiments. Journal of Allergy and Clinical Immunology 2010 126, 375-383DOI: (10.1016/j.jaci.2010.05.040) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions