Volume 56, Pages S254-S255 (July 1999)

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Volume 56, Pages S254-S255 (July 1999) Phosphotyrosine of macrophage by low-density lipoproteins from hemodialysis patients Miyahiko Sonobe, M.D, Keigo Kimura, Masanori Kishino, Yoshinori Tone, Yoichi Yamada, Masatoshi Mune, Susumu Yukawa, Iwao Nishide Kidney International Volume 56, Pages S254-S255 (July 1999) DOI: 10.1046/j.1523-1755.1999.07168.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Phosphotyrosine immunoblotting of human macrophage proteins. Human macrophages were either unstimulated (lane 1) or treated with native low-density lipoprotein (LDL; lanes 2 and 4) and HD-LDL (lanes 3 and 5) for the indicated times. Lysates were divided into Triton-soluble and -insoluble fractions, as described in the Methods section. Proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotted with antiphosphotyrosine antibody, and visualized by enhanced chemiluminescence. Kidney International 1999 56, S254-S255DOI: (10.1046/j.1523-1755.1999.07168.x) Copyright © 1999 International Society of Nephrology Terms and Conditions