A novel system for in vitro maturation of human oocytes

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A novel system for in vitro maturation of human oocytes Natalie A Cekleniak, M.D., Catherine M.H Combelles, B.S., David A Ganz, B.A., Jingly Fung, Ph.D., David F Albertini, Ph.D., Catherine Racowsky, Ph.D. Fertility and Sterility Volume 75, Issue 6, Pages 1185-1193 (June 2001) DOI: 10.1016/S0015-0282(01)01789-7

Figure 1 Germinal vesicle oocytes cultured in TC199-S (T: GV) and P1-S (P1: GV) that matured after 24 hours in culture to metaphase II (T: MII and P1: MII). Arrows indicate the germinal vesicle (magnification, ×400). Cekleniak. In vitro maturation of human oocytes. Fertil Steril. Fertility and Sterility 2001 75, 1185-1193DOI: (10.1016/S0015-0282(01)01789-7)

Figure 2 Organization of microtubules and chromosomes in human oocytes after in vitro maturation. Dual-labeled confocal images of three-dimensional reconstructions of meiotic spindle structures stained for tubulin (red) and histone H1 (green) are shown (78 oocytes were analyzed). (A), Typical bipolar metaphase II spindles with chromosomes aligned at the equator. (B), A bipolar spindle is shown, with chromosomes dispersed throughout the spindle structure. (C) and (D), Examples of nonbipolar spindles characterized by a central mass of microtubules surrounded by chromosomes. In all cases, chromosomes interact with the surface of microtubule aggregates (yellow areas denote sites of interaction). (scale bar = 10 μm). Cekleniak. In vitro maturation of human oocytes. Fertil Steril 2001. Fertility and Sterility 2001 75, 1185-1193DOI: (10.1016/S0015-0282(01)01789-7)