WASH cKO mice display a normal pancreatic development.

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WASH cKO mice display a normal pancreatic development. WASH cKO mice display a normal pancreatic development. A and B: Shown were the body weight and fasting blood glucose level in 8- and and 16-week-old WT and cKO mice. C: Real-time PCR quantification of mice pancreatic cytokeratin 19 (CK19), amylase, insulin, glucagon (Gcg), and somatostatin (SS) from WT and cKO mice was examined. D: Freshly isolated primary mouse islets from WT or cKO mice were cultured in RPMI-1640 medium. Photos showed dithizone (diphenylthiocarbazone, DTZ)-stained normal islets architecture highlighted with red dot line. Bars, 200 μm. E: Size of isolated islets was quantified by ImageJ. F: Pancreatic insulin protein was extracted from pancreas tissue extracts, measured by ELISA, and normalized with total protein level. Results were expressed as mean ± SEM. n = 6. wks, weeks. Li Ding et al. Diabetes 2019;68:377-386 ©2019 by American Diabetes Association