Muscles & Motion
Evolution of Movement 1) epitheliomuscular cells in Cnidarians epithelial cells w/ contractile protein arrays (actin & myosin) 2) epitheliomuscular cells sank below surface into connective tissues and became muscle
Bundles of muscle cells fuse together inside a covering of connective tissue Bundles of fused muscle cells form muiltinucleate muscel fibers The connective tissue covering extend past the muscle fibers to attach to bones
Regulatory Proteins Tropomyosin – blocks myosin binding sites on actin Troponin complex – move tropomyosin out of the way so myosin can bind to the actin ….activated by Ca
Myosin heads primed by ATP
Binding site blocked by tropomyocin Ca bonding to troponin Δ its shape Δ troponin moves tropomyocin out of binding site Myosin binds to actin
Myosin releases ADP and P and bends toward center of sarcomere Actin pulled toward center ATP binds myosin & resets myosin head http://www.dnatube.com/video/1306/Role-of-myosin-crossbridge-in-the-contraction-of-muscle
Neuromuscular Junctions CNS sends action potential down motor N Synaptic Vs release Ach (acetylcholine) Ach diffuses across synapse Ach binds to membrane receptors triggers action potential that spreads through muscle fiber along T-tubules T-tubules are infoldings of cell membrane http://www.dnatube.com/video/1950/Molecular-events-in-muscle-contraction http://msjensen.cehd.umn.edu/1135/Links/Animations/Flash/0010-swf_action_potenti.swf
Sarcoplasmic Reticulum (SR) Action Potentia in T-tubule activates SR http://www.sumanasinc.com/webcontent/animations/content/muscle.html 1. SR releases Ca into muscle fiber 2. Ca binds to Troponin 3. Troponin moves Tropomyosin 4. Muscle contracts http://www.youtube.com/watch?v=XoP1diaXVCI
Lack of Action Potential Sarcoplasmic Reticulum pumps Ca back in Low Ca levels in muscle fiber Tropomyosin blocks myosin binding sites on actin. Sarcomeres relax