Representative thermogram displaying the exothermic and endothermic events associated with the cooling and heating of a sample containing P1-stage Halobiotus crispae from Vellerup Vig, Denmark (20 ppt). Representative thermogram displaying the exothermic and endothermic events associated with the cooling and heating of a sample containing P1-stage Halobiotus crispae from Vellerup Vig, Denmark (20 ppt). The crystallization and melting temperatures were estimated as the onsets of peaks (–17.5°C and –4.5°C, respectively) using the DSC 7 software (see also Table 1). The very low crystallization temperatures measured exclude the presence of physiologically relevant ice-nucleating agents. Kenneth Agerlin Halberg et al. J Exp Biol 2009;212:2803-2811 ©2009 by The Company of Biologists Ltd