The spindle observation and its relationship with fertilization after intracytoplasmic sperm injection in living human oocytes  Wei-Hua Wang, Ph.D., Li Meng, Ph.D., Rickard J Hackett, Rudolf Odenbourg, Ph.D., David L Keefe  Fertility and Sterility  Volume 75, Issue 2, Pages 348-353 (February 2001) DOI: 10.1016/S0015-0282(00)01692-7

Figure 1 Spindles in living human oocytes imaged by using the polscope just before ICSI. (A), An oocyte with a spindle located just under the plasma membrane, near the polar body. (B), An oocyte with a spindle forming at 43° to the polar body. (C), An oocyte with a spindle that migrated to a deeper position (arrow) in the cytoplasm and that is forming at 42.5° to the polar body. Original magnification, ×300). pb=polar body. Wang. Spindle observation in living human eggs. Fertil Steril 2001. Fertility and Sterility 2001 75, 348-353DOI: (10.1016/S0015-0282(00)01692-7)

Figure 2 Spindles in aged human oocytes as seen with the polarization microscope (polscope) and confocal microscope. (A), A day 1 oocyte with a small spindle. The confocal microscopy image of this oocyte was nearly identical to the polscope image (insert); (B), Confocal microscope image of aged oocytes on day 2. (C), Confocal microscope image of aged oocytes on day 3. In the latter two images, no spindle can be seen in the oocytes and chromosomes are scattered throughout the cytoplasm (arrows). The green portions represent microtubules, red represents chromatin, and yellow represents the overlay of the green and red images. Original magnification, ×300). pb=polar body. Wang. Spindle observation in living human eggs. Fertil Steril 2001. Fertility and Sterility 2001 75, 348-353DOI: (10.1016/S0015-0282(00)01692-7)