Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

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Alteration of focal adhesion kinase expression in eutopic endometrium of women with endometriosis Lin Mu, Ph.D., Wei Zheng, Ph.D., M.D., Liang Wang, Ph.D., Xue-Jun Chen, M.D., Xiang Zhang, M.D., Jian-Hua Yang, Ph.D. Fertility and Sterility Volume 89, Issue 3, Pages 529-537 (March 2008) DOI: 10.1016/j.fertnstert.2007.03.060 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Immunohistochemical staining for FAK in the endometrium of women with and without endometriosis. Immunoreactivity of FAK was detected in human endometrium at both proliferative and secretory phases. (A, C) Tissue sections from women without endometriosis at proliferative and secretory phases of the cycle, respectively. (B, D) Tissue sections from women with endometriosis at proliferative and secretory phases, respectively. Original magnification, ×200. Fertility and Sterility 2008 89, 529-537DOI: (10.1016/j.fertnstert.2007.03.060) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Focal adhesion kinase protein expression in endometrial tissues was assessed by Western blotting. (A) The anti-FAK antibody detected a band at 125 kDa. Lane A, proliferative endometrium from controls. Lane B, proliferative endometrium from women with endometriosis. Lane C, secretory endometrium from controls. Lane D, secretory endometrium from women with endometriosis. (B) Normalized density was analyzed by using the internal β-actin as reference (means ± SD). aP=.020, compared with the normalized density of FAK proteins in secretory endometrial tissues of controls. Fertility and Sterility 2008 89, 529-537DOI: (10.1016/j.fertnstert.2007.03.060) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Expression of FAK mRNA was assessed by reverse-transcription PCR in endometrial samples. (A) A 620-bp product of FAK mRNA was visualized with ethidium bromide after agarose gel electrophoresis. Glyceraldehyde phosphate dehydrogenase (452 bp) was used as a control to assess the amount of RNA in each sample. Molecular DNA markers (lane M), proliferative endometrium from control (lane A), proliferative endometrium from women with endometriosis (lane B), secretory endometrium from controls (lane C), and secretory endometrium from women with endometriosis (lane D). (B) Quantification of FAK mRNA level was analyzed by using the internal glyceraldehyde phosphate dehydrogenase as reference (means ± SD). aP=.030, compared with the expression of FAK mRNA in secretory endometrial tissues of controls. Fertility and Sterility 2008 89, 529-537DOI: (10.1016/j.fertnstert.2007.03.060) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 4 There were positive correlations between the normalized densities of endometrial FAK proteins and the serum E2 levels at the proliferative phase (n = 50, r2 = .141, P=.003; A) and the ratio of serum E2 to P at the secretory phase (n = 52, r2= .117, P=.013; B). Fertility and Sterility 2008 89, 529-537DOI: (10.1016/j.fertnstert.2007.03.060) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions