Effect of the plastid translation inhibitor Spec on LR development

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Volume 23, Issue 20, Pages (October 2013)
Expression of the AREB1 Gene and Subcellular Localization of the AREB1 Protein.(A) Structure of AREB1 family proteins. Expression of the AREB1 Gene and.
Tissue expression pattern of SOS1.
Live-cell imaging of transcriptional activation following TSA treatment. Live-cell imaging of transcriptional activation following TSA treatment. (A) Frames.
Fig. 8. In vitro effect of CMPs on adult DSS-treated stomach explants.
Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.
Fig. 4. Expression of oxt is undetectable in the med12 mutant but is unaffected by treatment with Wnt inhibitors. Expression of oxt is undetectable in.
Fig. 4. smc3 regulates the expression of cx43 in regenerating fins.
BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.
Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.
Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.
Fig. 4. Seeding density modulates cell shape in both cell types
A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.
Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle
Fig. 4. A primary screen based on scrape closure
Fig. 6. Cross-section of the stomach wall and spiral intestine of the embryo, stained with PAS. (A) Surface of the stomach wall (SW) and ingested material.
Fig. 10. Ratiometric live imaging of di-4-ANEPPDHQ in growing pollen tubes.(A) Higher and lower membrane order distribution in control and BCD treated.
Fig. 1. Mitochondrial internalization in cardiomyocytes.
Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.
Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.
Fig. 1. Loss of PC following INT depletion
Fig. 1. Morphological and growth characterization of hBMCs and hPDCs
Fig. 2. Transfection and clonal selection of rat pluripotent stem cells to generate stable transgenic lines. Transfection and clonal selection of rat pluripotent.
Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.
Fig. 2. Salivary glands from RasV12-expressing larvae produce MMP1, release tissue fragments into the hemolymph and express apoptotic markers.Salivary.
Fig. 1. Pigmentation and melanophore counts of rainbow trout parr and smolt caudal fins.Pigmentation of (A) parr and (B) smolt. Pigmentation and melanophore.
eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots
Fig. 1. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and.
Fig. 1. Ovarian cancer spheroids can bud from a monolayer
The initial targeting of Sec61b mRNA to the ER is partially dependent on ribosomes and translation. The initial targeting of Sec61b mRNA to the ER is partially.
Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2
Fig. 6. LR phenotype of plastid translation-defective mutants with/without Spec. LR phenotype of plastid translation-defective mutants with/without Spec.
Fig. 4. Detection of dFMR1 mRNA in dFMRP granules by FISH
Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.
Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.
Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.
Fig. 7. The sma gene is necessary for ampA mRNA accumulation and its loss phenocopies some ampA effects.(A) RT-PCR showing levels of ampA transcripts in.
The coding potential of GFP-Sec61b is not required for its localization to the ER. (A) COS7 cells were transfected with plasmid encoding various GFP-tagged.
Fig. 5. Morphological changes of the N
Fig. 5. The bulk of Cep135 localizes distantly from Sas-6 and STIL
Fig. 6. Comparison of Plk4 with Sas-6 localization
Fig. 5. GFP fluorescence colocalization of Gcn5.
Fig. 2. The localisation of integrins β1 and α6 in the hES cells grown in a colony and as a single-cell culture. hES cells were harvested manually as small.
Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.
Fig. 7. Lhx1-RNAi reduces the eye size
Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.
Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.
Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.
GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.
Fig. 6. STK35 KO mice show ovary defects.
BMP signalling is dispensable for early gastruloid patterning.
Fig. 2. Fluorescent images indicating the cytoskeleton of human bone marrow-derived mesenchymal stem cells (hBMSCs) subjected to cyclic stretching. Fluorescent.
Fig. 4. SMXL6 is degraded in response to SL treatment.
Statistical chart of significantly differentially expressed genes
Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.
Fig. 2. RFC3 function in chloroplasts.
Tau–RFP–RBPs colocalize with mRNA on microtubules and lead to the wetting of stress granules on microtubules. Tau–RFP–RBPs colocalize with mRNA on microtubules.
Fig. 2. Non-homogeneous subcellular distribution of Vangl2 along the anteroposterior axis. Non-homogeneous subcellular distribution of Vangl2 along the.
Fig. 2. iPSCs produce functional osteoblasts.
Ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis. ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis.
CENP-F staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-F staining in prometaphase and metaphase spermatocytes from B6.
Fig. 2. Acetylation stiffens primary cilia.
Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.
Depletion of COPI protein inhibits cis to trans cisternal maturation.
Fig. 5. Co-expression analyses of disease mutations in YFP-RPGRIP1α1 with wild-type RFP-RPGR1–19 or RFP-RPGRORF15 in COS7 cells.YFP-RPGRIP1α1 with disease-associated.
WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.
Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP
* Supplementary Fig. 1 (Yamanaka et al.) * * * ** ** ** * * **
NRG2 Affects Nitrate Accumulation and Uptake
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Fig. 5. Effect of the plastid translation inhibitor Spec on LR development. Effect of the plastid translation inhibitor Spec on LR development. (A) mPS-PI-stained LRs of L. er plants grown on medium containing various Spec concentrations. Arrowheads indicate the position of the QC cells, brackets indicate the area occupied by misaligned or stratified cells. (B) Relative expression levels of WOX5, PLT3, and PLT7 in Spec-treated plants (n=3). (C) Distribution of GFP fluorescence (green) in WOX5p::GFP merged with bright field images. (D,E) Relative transcript levels of plastid ribosomal RNAs (D) and plastid-encoded genes (E) in roots (n=3). (F) Correlation analysis of changes in expression (Log2) in plastid-encoded genes resulting from the rfc3 mutation and 4 mg l−1 Spec treatment. R, Pearson's correlation value. Statistical analyses were carried out as described in Fig. 3. Scale bars: 50 µm (A,C). Miyuki T. Nakata et al. Biology Open 2018;7:bio028175 © 2018. Published by The Company of Biologists Ltd