Activation of integrin receptors is required for growth factor–induced smooth muscle cell dysfunction Kyotaro Mawatari, MD, Bo Liu, PhD, K.Craig Kent,

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Activation of integrin receptors is required for growth factor–induced smooth muscle cell dysfunction Kyotaro Mawatari, MD, Bo Liu, PhD, K.Craig Kent, MD Journal of Vascular Surgery Volume 31, Issue 2, Pages 375-381 (February 2000) DOI: 10.1016/S0741-5214(00)90167-8 Copyright © 2000 Society of Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Effect of inhibitory integrin antibodies on SMC DNA synthesis induced by PDGF-BB. DNA synthesis (24 hours) as measured by 3H-thymidine incorporation following stimulation of quiescent vascular SMCs with PDGF-BB (5 ng/mL) in the presence of antibodies to αvβ3, α2β1, α2, β1, and IgG control (dilutions 1:100). Results are expressed as percent of IgG control ± SD. Experiments were performed in triplicate and repeated three times with use of separate cell cultures. Data from a representative example are shown (*P <.05 compared with control). Journal of Vascular Surgery 2000 31, 375-381DOI: (10.1016/S0741-5214(00)90167-8) Copyright © 2000 Society of Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Effect of inhibitory integrin antibodies on SMC DNA synthesis induced by EGF. DNA synthesis (24 hours) as measured by 3H-thymidine incorporation following stimulation of quiescent vascular SMCs with EGF (20 ng/mL) in the presense of antibodies to αvβ3, α2β1, α2, β1, and IgG control (dilutions 1:100). Results are expressed as percent of IgG control ± SD. Experiments were performed in triplicate and repeated three times with use of separate cell cultures. Data from a representative example are shown (*P <.05 compared with control). Journal of Vascular Surgery 2000 31, 375-381DOI: (10.1016/S0741-5214(00)90167-8) Copyright © 2000 Society of Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Effect of inhibitory integrin antibodies on SMC DNA synthesis induced by bFGF. DNA synthesis (24 hours) as measured by 3H-thymidine incorporation following stimulation of quiescent vascular SMCs with bFGF (20 ng/mL) in the presense of antibodies to αvβ3, α2β1, α2, β1, and IgG control (dilutions 1:100). Results are expressed as percent of IgG control ± SD. Experiments were performed in triplicate and repeated three times with use of separate cell cultures. Data from a representative example are shown (*P <.05 compared with control). Journal of Vascular Surgery 2000 31, 375-381DOI: (10.1016/S0741-5214(00)90167-8) Copyright © 2000 Society of Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Effect of inhibitory integrin antibodies on SMC fibronectin production induced by TGF-β and EGF. Fibronectin production as measured by Western blotting following 72 hours stimulation of quiescent vascular SMCs with TGF-β (20 ng/mL) (upper panel) and EGF (100 ng/mL) (lower panel) in the presence of antibodies to αvβ3, α2β1, α2, β1, and IgG control (dilutions 1:100). Fn was assayed in the cell medium with use of an antibody specific for the 220-kDa subunit. All experiments were repeated three times with cells from three separate cultures. Data from a representative example are shown. Journal of Vascular Surgery 2000 31, 375-381DOI: (10.1016/S0741-5214(00)90167-8) Copyright © 2000 Society of Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions