Crim1 colocalizes with integrin and activates the integrin-FAK-ERK signaling pathway. Crim1 colocalizes with integrin and activates the integrin-FAK-ERK.

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Crim1 colocalizes with integrin and activates the integrin-FAK-ERK signaling pathway. Crim1 colocalizes with integrin and activates the integrin-FAK-ERK signaling pathway. (A) Crim1 and β1 integrin colocalize at the tip of lamellipodia (A′, arrows) and filopodia (A″, arrowhead) in 21EM15 cells. (B) Immunocytochemical detection of active (9EG7) β1 integrin on the cell surface of 21EM15 lens cells transfected with HA-tagged Crim1-FL vector (top row), Crim1-ED (middle row) or Crim1-RRD (bottom row). Arrows point to active integrin staining. (C) Expression constructs representing Myc-tagged full-length Crim1 (FL), Crim1 extracellular domain only (ED), Crim1 lacking the intracellular domain (ΔID), and Crim1 carrying RGD in place of RRD (Crim1-RRD) were transfected into HEK 293T cells for 48 h and then western blotted with the indicated antibodies. pFAKY397 and pERK are induced by FL and ΔID, whereas RRD induces pERK but not pFAKY397. Ctrl is vector only. Images shown are representative of three independent experiments. Scale bars: 10 μm. Ying Zhang et al. Development 2016;143:356-366 © 2016. Published by The Company of Biologists Ltd